抗IL-10抗体(Anti-IL-10, Porcine, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Porcine
Label	Biotin
Immunogen	E. coli-derived recombinant porcine IL-10Ser19-Asn175Accession # Q29055
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects porcine IL-10 in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Porcine IL‑10 (Catalog # 693-PI)

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Related Product & Information

Background

IL-10

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Background: IL-10 IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). The human homologue of murine IL-10 was subsequently cloned by cross-hybridization. Human and murine IL-10 are 81% and 73% homologous at the nucleotide and amino acid level, respectively. Both murine and human IL-10 are also highly homologous with a previously uncharacterized open reading frame in the Epstein-Bar virus (EBV) genome, BCRF1. The BCRF1 gene product is now designated viral IL-10. Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1+ (CD5+) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 is 71% and 78% homologous with mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

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Citations

1. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
   Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
   Infect. Immun., 2017;85(4):.
   Species: Porcine
   Sample Type: Serum
   Application: ELISA detection


2. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
   Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
   Intensive Care Med, 2007;33(6):1085-93.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development

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