抗TNF-α抗体(Anti-TNF-α, Rat, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27731
TNF-αに対する抗体(Anti-TNF-α, Rat, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2026年04月06日 18時15分現在]
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[在庫・価格 ：2026年04月06日 18時15分現在]
Anti-TNF-α, Rat, Goat-Poly, Biotin
文献数： 6
- 商品コード：BAF510
- メーカー：RSD
- 包装：50μg
- 価格：¥91,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat TNF-α サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB510-500または#MAB510R-100，スタンダードとして#510-RT-010を用いる。 別名：APC1 protein Genbank No： 7124 Protein Accession No： P16599 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Rat | 免疫動物 | Goat |
| 交差性 | Rat | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant rat TNF-alpha (R&D Systems, Catalog # 510-RT)Leu80-Leu235Accession # P16599 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rat TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 2.5% cross‑reactivity with rmTNF-a is observed and less than 0.2% cross‑reactivity with rhTNF-a and rpTNF-a is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Rat TNF‑ alpha (Catalog # 510-RT) |
| Rat TNF-alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat TNF‑ alpha Antibody (Catalog #MAB510 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat TNF‑ alpha Biotinylated Antibody (Catalog #BAF510 ) |
| ELISA Standard | Recombinant Rat TNF-alpha Protein (Catalog #510-RT ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: TNF-alpha | Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69% - 76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11). |
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References
| Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. | |
| Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. | |
| Estler, H.C. et al. (1992) Biol. Chem. Hoppe-Seyler 373:271. | |
| Tang, P. et al. (1996) Biochemistry 35:8216. | |
| Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. | |
| Black, R.A. et al. (1997) Nature 385:729. | |
| Moss, M.L. et al. (1997) Nature 385:733. | |
| Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. | |
| Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. | |
| Romanatto, T. et al. (2007) Peptides 28:1050. | |
| Hector, J. et al. (2007) Horm. Metab. Res. 39:250. | |
| Long Name: | Tumor Necrosis Factor alpha |
| Entrez Gene IDs: | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
| Alternate Names: | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
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Related Research Areas
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Rat |
| Other Experimental Details | This antibody was paired with MAB510 to build an ELISA to measure rat TNF-alpha in plasma samples. |
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Related Product & Information
| Background | TNF-alpha |
|---|---|
| background_content | Background: TNF-alpha Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69% - 76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11). |
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Citations
- Ulva lactuca polysaccharides prevent Wistar rat breast carcinogenesis through the augmentation of apoptosis, enhancement of antioxidant defense system, and suppression of inflammation
Authors: GF Abd-Ellate, OM Ahmed, ES Abdel-Rehe, AZ Abdel-Hami
Breast Cancer (Dove Med Press), 2017;9(0):67-83.
Species: Rat
Sample Type: Serum
Application: ELISA detection - Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
Infect. Immun., 2008;76(8):3577-86.
Species: Rat
Sample Type: BALF
Application: ELISA Development - In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Authors: Roche M, Diamond M, Finn DP
J. Neuroimmunol., 2006;181(1):57-67.
Species: Rat
Sample Type: Plasma
Application: ELISA Development - TNFalpha-induced MMP-9 promotes macrophage recruitment into injured peripheral nerve.
Authors: Shubayev VI, Angert M, Dolkas J, Campana WM, Palenscar K, Myers RR
Mol. Cell. Neurosci., 2006;31(3):407-15.
Species: Rat
Sample Type: Cell Lysates
Application: WB
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