抗IL-4抗体(Anti-IL-4, Rat, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27727
IL-4に対する抗体(Anti-IL-4, Rat, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年07月15日 00時00分現在]
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Anti-IL-4, Rat, Goat-Poly, Biotin |
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[在庫・価格 ：2025年07月15日 00時00分現在]
Anti-IL-4, Rat, Goat-Poly, Biotin
文献数： 3
- 商品コード：BAF504
- メーカー：RSD
- 包装：50μg
- 価格：¥88,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat IL-4 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB504-500，スタンダードとして#504-RL-005を用いる。 別名：B cell growth factor 1 Genbank No： 3565 Protein Accession No： P20096 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Rat | 免疫動物 | Goat |
| 交差性 | Rat | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant rat IL‑4Cys25-Ser147Accession # P20096 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rat IL-4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse IL-4 and recombinant human IL-4 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Rat IL‑4 (Catalog # 504-RL) |
| Rat IL-4 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat IL‑4 Antibody (Catalog #MAB504 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat IL‑4 Biotinylated Antibody (Catalog #BAF504 ) |
| ELISA Standard | Recombinant Rat IL-4 Protein (Catalog #504-RL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-4 | Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14). |
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References
| Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. | |
| Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1. | |
| McKnight, A.J. et al. (1991) Eur. J. Immunol. 21:1187. | |
| Redfield, C. et al. (1991) Biochemistry 30:11029. | |
| Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141. | |
| Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451. | |
| Mosman, T.R. et al. (1987) J. Immunol. 138:1813. | |
| Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237. | |
| Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701. | |
| Paludan, S.R. (1998) Scand. J. Immunol. 48:459. | |
| Corthay, A. (2006) Scand. J. Immunol. 64:93. | |
| Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. | |
| Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. | |
| Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303. | |
| Long Name: | Interleukin 4 |
| Entrez Gene IDs: | 3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit) |
| Alternate Names: | B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra |
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Related Research Areas
| Common gamma Chain Receptor Family | ||||||||||
| Cytokines and Receptors in Allergy and Asthma | ||||||||||
| Cytokines and Receptors in Angiogenesis | ||||||||||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||||||||||
| Dendritic Cell Development | ||||||||||
| gamma delta T Cells | ||||||||||
| Group 2 ILCs | ||||||||||
| Inflammatory Mediators | ||||||||||
| Multiple Sclerosis and Myelin Related Molecules | ||||||||||
| Natural Killer T (NKT) Cells | ||||||||||
| Regulatory T Cells (Tregs) | ||||||||||
| T Cell Cytokine Signaling | ||||||||||
| Th2 Cells | ||||||||||
| Th9 Cells | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ELISA | Rat | Anonymous | 11/08/2017 | Image Details |
 ELISA: Rat IL-4 Biotinylated Antibody [BAF504]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum |
| Species | Rat |
| Other Experimental Details | This antibody was used as the detection paired with MAB504 as a capture in an ELISA to measure rat IL-4. Both antibodies worked well in this application. |
追加しました。
Related Product & Information
| Background | IL-4 |
|---|---|
| background_content | Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14). |
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Citations
- Overexpression of interleukin-13 induces minimal-change-like nephropathy in rats.
Authors: Lai KW, Wei CL, Tan LK, Tan PH, Chiang GS, Lee CG, Jordan SC, Yap HK
J. Am. Soc. Nephrol., 2007;18(5):1476-85.
Species: Rat
Sample Type: Serum
Application: ELISA Development
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