抗CD14抗体(Anti-CD14, Human, Sheep-Poly, Biotin antibody)

掲載日情報:2021/01/28 現在Webページ番号:27624

CD14に対する抗体(Anti-CD14, Human, Sheep-Poly, Biotin )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年02月16日 00時00分現在]

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Anti-CD14, Human, Sheep-Poly, Biotin
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 6
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説明文
マッチドペア:Human CD14 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB3833-500,スタンダードとして#383-CD-050を用いる。
別名:CD14
Genbank No: 929
Protein Accession No: P08571
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Sheep クラス IgG 標識 Biotin
交差性 Human 適用 ELISA,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Myeloid-derived Suppressor Cell Marker
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2025年02月16日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-CD14, Human, Sheep-Poly, Biotin

文献数: 6

説明文 マッチドペア:Human CD14 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB3833-500,スタンダードとして#383-CD-050を用いる。
別名:CD14
Genbank No: 929
Protein Accession No: P08571
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Sheep
交差性 Human 適用 ELISA,IHC,Western Blot
標識 Biotin 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Myeloid-derived Suppressor Cell Marker
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityHuman
LabelBiotin
ImmunogenChinese hamster ovary cell line CHO-derived recombinant human CD14Thr20-Cys352Accession # P08571
SourcePolyclonal Sheep IgG
PurificationAntigen Affinity-purified
SpecificityDetects human CD14 in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse CD14 and Lipopolysaccharide is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot0.1 µg/mLRecombinant Human CD14 (Catalog # 383-CD)
Immunohistochemistry5-15 µg/mLSee below
Human CD14 Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)2-8 µg/mL Human CD14 Antibody (Catalog #MAB3833 )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Human CD14 Biotinylated Antibody (Catalog #BAF383 )
ELISA Standard  Recombinant Human CD14 Protein (Catalog #383-CD )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunohistochemistry Immunohistochemistry CD14 Antibody [Biotin]
click image to view larger
CD14 in Human Lymph Node.CD14 was detected in immersion fixed paraffin-embedded sections of human lymph node using 10 µg/mL Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS020) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

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References

Wright, S.D. et al. (1990) Science 249:1431.
Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
Beutler, B. (2000) Current Opinion in Immunology 12:20.
Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs:929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names:CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

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Related Research Areas

Show
Common Macrophage Markers
Conventional/Classical Dendritic Cells
Immune Regulatory Molecules on Endothelial Cells
Inflammatory Mediators
Inflammatory/Monocyte-derived Dendritic Cells
Langerhans and Dermal Dendritic Cells
Leukocyte Receptors/Markers in Allergy and Asthma
Monocyte Markers
Myeloid Lineage Markers
Myeloid-derived Suppressor Cells (MDSC)
Plasmacytoid Dendritic Cells
Toll-Like Receptors
Uptake of Amyloid beta by Glia Cells
Uptake of Amyloid beta by Macrophages
  1. Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
    Authors: Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D
    PLoS ONE, 2016;11(1):e0148073.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
ImagesRatingsApplicationsSpeciesReviewed ByDateDetails
Excellent
 Flow Human Anonymous 07/27/2016

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Summary

Application Flow Cytometry
Sample Tested Peripheral blood cells
Species Human


Immunohistochemistry
CD14 in Human Lymph Node.
CD14 was detected in immersion fixed paraffin-embedded sections of human lymph node using 10 µg/mL Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS020) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.


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Related Product & Information

BackgroundCD14
background_contentBackground:
CD14
CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
    Authors: Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D
    PLoS ONE, 2016;11(1):e0148073.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development

  2. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Type: Serum
    Application: ELISA Microarray Development

  3. Killing the messenger in the nick of time: persistence of breast milk sCD14 in the neonatal gastrointestinal tract.
    Authors: Blais DR, Harrold J, Altosaar I
    Pediatr. Res., 2006;59(3):371-6.
    Species: Human
    Sample Type: Milk
    Application: WB

  4. Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation.
    Authors: Boissy P, Andersen TL, Abdallah BM, Kassem M, Plesner T, Delaisse JM
    Cancer Res., 2005;65(21):9943-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Immunopurification



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