抗CD14抗体(Anti-CD14, Human, Sheep-Poly, Biotin antibody)
掲載日情報:2021/01/28 現在Webページ番号:27624
CD14に対する抗体(Anti-CD14, Human, Sheep-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年02月16日 00時00分現在]
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Anti-CD14, Human, Sheep-Poly, Biotin |
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[在庫・価格 :2025年02月16日 00時00分現在]
Anti-CD14, Human, Sheep-Poly, Biotin
文献数: 6
- 商品コード:BAF383
- メーカー:RSD
- 包装:50μg
- 価格:¥119,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Human CD14 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB3833-500,スタンダードとして#383-CD-050を用いる。 別名:CD14 Genbank No: 929 Protein Accession No: P08571 |
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法規制等 | |||
保存条件 | -20℃ | 法規備考 | |
抗原種 | Human | 免疫動物 | Sheep |
交差性 | Human | 適用 | ELISA,IHC,Western Blot |
標識 | Biotin | 性状 | Antigen Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Polyclonal | フォーマット | |
掲載カタログ |
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製品記事 | 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) Myeloid-derived Suppressor Cell Marker |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Human |
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Label | Biotin |
Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human CD14Thr20-Cys352Accession # P08571 |
Source | Polyclonal Sheep IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects human CD14 in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse CD14 and Lipopolysaccharide is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.1 µg/mL | Recombinant Human CD14 (Catalog # 383-CD) |
Immunohistochemistry | 5-15 µg/mL | See below |
Human CD14 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CD14 Antibody (Catalog #MAB3833 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CD14 Biotinylated Antibody (Catalog #BAF383 ) |
ELISA Standard | Recombinant Human CD14 Protein (Catalog #383-CD ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Immunohistochemistry |
![]() click image to view larger | CD14 in Human Lymph Node.CD14 was detected in immersion fixed paraffin-embedded sections of human lymph node using 10 µg/mL Sheep Anti-Human CD14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF383) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS020) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: CD14 | CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14. |
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References
Wright, S.D. et al. (1990) Science 249:1431. | |
Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744. | |
Beutler, B. (2000) Current Opinion in Immunology 12:20. | |
Stelter, F. (2000) Chem. Immunol. 74:25. | |
Entrez Gene IDs: | 929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine) |
Alternate Names: | CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein |
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Related Research Areas
Common Macrophage Markers | ||||||||
Conventional/Classical Dendritic Cells | ||||||||
Immune Regulatory Molecules on Endothelial Cells | ||||||||
Inflammatory Mediators | ||||||||
Inflammatory/Monocyte-derived Dendritic Cells | ||||||||
Langerhans and Dermal Dendritic Cells | ||||||||
Leukocyte Receptors/Markers in Allergy and Asthma | ||||||||
Monocyte Markers | ||||||||
Myeloid Lineage Markers | ||||||||
Myeloid-derived Suppressor Cells (MDSC) | ||||||||
Plasmacytoid Dendritic Cells | ||||||||
Toll-Like Receptors | ||||||||
Uptake of Amyloid beta by Glia Cells | ||||||||
Uptake of Amyloid beta by Macrophages | ||||||||
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Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
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Excellent | Flow | Human | Anonymous | 07/27/2016 |
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Summary
Application | Flow Cytometry |
Sample Tested | Peripheral blood cells |
Species | Human |
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Related Product & Information
Background | CD14 |
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background_content | Background: CD14 CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14. |
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Citations
- Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
Authors: Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D
PLoS ONE, 2016;11(1):e0148073.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Species: Human
Sample Type: Serum
Application: ELISA Microarray Development - Killing the messenger in the nick of time: persistence of breast milk sCD14 in the neonatal gastrointestinal tract.
Authors: Blais DR, Harrold J, Altosaar I
Pediatr. Res., 2006;59(3):371-6.
Species: Human
Sample Type: Milk
Application: WB - Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation.
Authors: Boissy P, Andersen TL, Abdallah BM, Kassem M, Plesner T, Delaisse JM
Cancer Res., 2005;65(21):9943-52.
Species: Human
Sample Type: Whole Cells
Application: Immunopurification
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