抗LAP抗体(Anti-LAP, TGF-β1, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP TGF‑ beta 1 and Chinese hamster ovary cell line CHO-derived recombinant human LAP TGF‑ beta 1Leu30-Ser390Accession # P01137
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human LAP TGF-beta 1 in Western blots. In this format, less than 1% cross-reactivity with mature recombinant human (rh) TGF‑ beta 1, porcine TGF‑ beta 2, rhTGF‑ beta 3, and recombinant amphibian TGF‑ beta 5 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP)

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Related Product & Information

Background

LAP (TGF-beta 1)

background_content

Background: LAP (TGF-beta 1) TGF-beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta  superfamily. TGF-  beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N‑terminal 249 aa (aa 30-278) latency-associated peptide (LAP) and a C-terminal 112 aa (aa 279-390) mature TGF-  beta 1 (4-6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non‑covalently associated after secretion, forming the small latent TGF-beta 1 complex (4-8). Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5‑7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1 (10).

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Citations

1. Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells.
   Authors: Huygens C, Lienart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, Lucas S
   J Biol Chem, 2015;290(33):20105-16.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
   Authors: Oida T, Weiner HL
   J. Immunol., 2012;185(6):3529-35.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Involvement of regulatory T cells in the immunosuppression characteristic of patients with paracoccidioidomycosis.
   Authors: Ferreira MC, de Oliveira RT, da Silva RM, Blotta MH, Mamoni RL
   Infect. Immun., 2010;78(10):4392-401.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens.
   Authors: Burton OT, Gibbs S, Miller N, Jones FM, Wen L, Dunne DW, Cooke A, Zaccone P
   Eur. J. Immunol., 2010;40(8):2221-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg.
   Authors: Stockis J, Colau D, Coulie PG, Lucas S
   Eur. J. Immunol., 2009;39(12):3315-22.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. T-cell-expressed proprotein convertase furin is essential for maintenance of peripheral immune tolerance.
   Authors: Pesu M, Watford WT, Wei L, Xu L, Fuss I, Strober W, Andersson J, Shevach EM, Quezado M, Bouladoux N, Roebroek A, Belkaid Y, Creemers J, O'Shea JJ
   Nature, 2008;455(7210):246-50.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
   Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
   J. Immunol., 2004;172(9):5753-64.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Tissue Homogenates
   Application: WB


8. Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA.
   Authors: La Cava A, Ebling FM, Hahn BH
   J. Immunol., 2004;173(5):3542-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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