抗TNF-α抗体(Anti-TNF-α, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27486
TNF-αに対する抗体(Anti-TNF-α, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-TNF-α, Human, Goat-Poly, Biotin |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-TNF-α, Human, Goat-Poly, Biotin
文献数： 38
- 商品コード：BAF210
- メーカー：RSD
- 包装：50μg
- 価格：¥120,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human TNF-α サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB610-500または#MAB610R-100，スタンダードとして#210-TA-020を用いる。 別名：APC1 protein Genbank No： 7124 Protein Accession No： P01375 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,IC,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant human TNF-alpha (R&D Systems, Catalog # 210-TA)Val77-Leu233Accession # P01375 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) TNF‑ beta, recombinant mouse (rm) TNF-alpha, recombinant rat TNF‑ alpha, recombinant porcine TNF-alpha, rhsTNF RII, rmsTNF RI, and rmsTNF RII is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human TNF‑ alpha (Catalog # 210-TA) |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Human TNF-alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human TNF‑ alpha Antibody (Catalog #MAB610R ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human TNF‑ alpha Biotinylated Antibody (Catalog #BAF210 ) |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
![Immunocytochemistry TNF-alpha Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/TNFalpha_BAF210_Immunocytochemistry__Immunofluorescence_19771.jpg) click image to view larger | TNF‑ alpha in Human PBMCs. TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF‑ alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF210) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: TNF-alpha | Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11). |
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References
| Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. | |
| Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. | |
| Pennica, D. et al. (1984) Nature 312:724. | |
| Tang, P. et al. (1996) Biochemistry 35:8216. | |
| Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. | |
| Black, R.A. et al. (1997) Nature 385:729. | |
| Moss, M.L. et al. (1997) Nature 385:733. | |
| Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. | |
| Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. | |
| Romanatto, T. et al. (2007) Peptides 28:1050. | |
| Hector, J. et al. (2007) Horm. Metab. Res. 39:250. | |
| Long Name: | Tumor Necrosis Factor alpha |
| Entrez Gene IDs: | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
| Alternate Names: | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
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Related Research Areas
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Summary
| Application | Immunohistochemistry |
| Sample Tested | Tumor cell lyastes |
| Species | Human |
| Immunocytochemistry | |
|---|---|
 | TNF‑ alpha in Human PBMCs. TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF‑ alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF210) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Background | TNF-alpha |
|---|---|
| background_content | Background: TNF-alpha Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Transmembrane TNF-dependent uptake of anti-TNF antibodies
Authors: A Deora, S Hegde, J Lee, CH Choi, Q Chang, C Lee, L Eaton, H Tang, D Wang, D Lee, M Michalak, M Tomlinson, Q Tao, N Gaur, B Harvey, S McLoughlin, B Labkovsky, T Ghayur
MAbs, 2017;0(0):0.
Species: Human
Sample Type: Protein
Application: WB - Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
PLoS ONE, 2017;12(4):e0175712.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils
Authors: SA Castro, R Collighan, PA Lambert, IH Dias, P Chauhan, CE Bland, I Milic, MR Milward, PR Cooper, A Devitt
Cell Death Dis, 2017;8(3):e2644.
Species: Human
Sample Type: Cell Culture Supernates - Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcgammaR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment.
Authors: Matt P, Lindqvist U, Kleinau S
PLoS ONE, 2015;10(9):e0137474.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
Diabetologia, 2011;54(10):2713-23.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - Protection from RNA and DNA viruses by IL-32.
Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
J. Immunol., 2011;186(7):4110-8.
Species: Human
Sample Type: Cell Culture Supernates
Application: Electrochemiluminescence - Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009;87(12):1814-20.
Species: Human
Sample Type: Urine
Application: Antibody Array Development - Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009;183(9):5799-806.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
Clin. Exp. Allergy, 2008;38(4):594-601.
Species: Human
Sample Type: BALF
Application: ELISA Development - Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
J. Immunol., 2008;180(4):2553-61.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
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