抗IL-5抗体(Anti-IL-5, Human, Rat-Mono, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27433
IL-5に対する抗体(Anti-IL-5, Human, Rat-Mono, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年07月01日 18時15分現在]
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Anti-IL-5, Human, Rat-Mono(JES1-5A10), Biotin |
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[在庫・価格 ：2025年07月01日 18時15分現在]
Anti-IL-5, Human, Rat-Mono(JES1-5A10), Biotin
文献数： 4
- 商品コード：BAM6051
- メーカー：RSD
- 包装：250μg
- 価格：¥95,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human IL-5 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB405-500または#MAB405R-100，スタンダードとして#205-IL-005を用いる。 別名：BCDF mu クローン：JES1-5A10 Genbank No： 3567 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Rat |
| 交差性 | Human | 適用 | ELISA |
| 標識 | Biotin | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Biotin |
| Immunogen | COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human IL-5 |
| Source | Monoclonal Rat IgG2A Clone # JES1-5A10 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects recombinant human IL-5 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL‑3, rhGM-CSF, or recombinant mouse IL-5 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Human IL-5 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human/Mouse IL‑5 Antibody (Catalog #MAB405R ) |
| ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Human IL‑5 Biotinylated Antibody (Catalog #BAM6051 ) |
| ELISA Standard | Recombinant Human IL-5 Protein (Catalog #205-IL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-5 | Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross‑reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF. IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high‑affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high‑affinity receptors for GM‑CSF and IL-3. |
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| Long Name: | Interleukin 5 | |
| Entrez Gene IDs: | 3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline) | |
| Alternate Names: | BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I | |
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Related Research Areas
| Common beta Chain Receptor Family | ||
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
| gamma delta T Cells | ||
| Granulocyte Markers | ||
| Group 2 ILCs | ||
| Inflammatory Mediators | ||
| T Cell Cytokine Signaling | ||
| Th2 Cells | ||
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Related Product & Information
| Background | IL-5 |
|---|---|
| background_content | Background: IL-5 Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross‑reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF.IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high‑affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high‑affinity receptors for GM‑CSF and IL-3. |
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Citations
- Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007;329(1):214-8.
Species: Human
Sample Type: Serum
Application: Luminex Development - Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007;53(11):2010-2.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006;120(2):220-31.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
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