抗IFN-γ抗体(Anti-IFN-γ, Rhesus Macaque, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：27401
IFN-γに対する抗体(Anti-IFN-γ, Rhesus Macaque, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-IFN-γ, Rhesus Macaque, Goat-Poly |
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Anti-Rhesus Macaque IFN-gamma Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IFN-γ, Rhesus Macaque, Goat-Poly
文献数： 0
- 商品コード：AF961
- メーカー：RSD
- 包装：100μg
- 価格：¥99,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rhesus Macaque IFN-γ サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF961，スタンダードとして#961-RM-025を用いる。 別名：IFG Genbank No： 3458 Protein Accession No： P63310 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Rhesus Macaque | 免疫動物 | Goat | ||||
| 交差性 | Rhesus Macaque | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Rhesus Macaque IFN-gamma Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF961-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：IFG Genbank No： 3458 Protein Accession No： P63310 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Rhesus Macaque | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rhesus Macaque |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant rhesus macaque IFN-gamma (R&D Systems, Catalog # 961-RM)Gln24-Gln165Accession # P63310 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rhesus macaque IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 30% cross-reactivity with recombinant human IFN‑ gamma is observed and less than 0.1% cross-reactivity with recombinant mouse IFN‑ gamma, recombinant rat IFN‑ gamma, recombinant porcine IFN‑ gamma, recombinant canine IFN‑ gamma, recombinant cotton rat IFN‑ gamma, and recombinant feline IFN‑ gamma is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Rhesus Macaque IFN-gamma (Catalog # 961-RM) |
| Rhesus Macaque IFN-gamma Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Rhesus Macaque IFN‑ gamma Antibody (Catalog #AF961 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rhesus Macaque IFN-gamma Biotinylated Antibody (Catalog #BAF961 ) |
| ELISA Standard | Recombinant Rhesus Macaque IFN-gamma Protein (Catalog #961-RM ) | |
| Neutralization | Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 1.5-5.0 µg/mL in the presence of 200 ng/mL Recombinant Rhesus Macaque IFN‑ gamma. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
 click image to view larger | IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Primate IFN‑ gamma Antibody.Recombinant Rhesus Macaque IFN‑ gamma (Catalog # 961-RM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN‑ gamma (200 ng/mL) is neutralized (green line) by increasing concentrations of Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961). The ND50 is typically 1.5-5.0 µg/mL. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IFN-gamma | Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rhesus IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid sequence identity with human IFN-gamma, 57-66% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37-44% with cotton rat, mouse, and rat IFN‑ gamma. IFN‑ gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). | |
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References
| Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. | |
| Pestka, S. et al. (2004) Immunol. Rev. 202:8. | |
| Villinger, F. et al. (1995) J. Immunol. 155:3946. | |
| Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401. | |
| Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. | |
| Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. | |
| McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. | |
| Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. | |
| Kelchtermans, H. et al. (2008) Trends Immunol. 29:479. | |
| Long Name: | Interferon gamma |
| Entrez Gene IDs: | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| Alternate Names: | IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma |
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Related Research Areas
| Neutralization | |
|---|---|
 | IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Primate IFN‑ gamma Antibody. Recombinant Rhesus Macaque IFN‑ gamma (Catalog # 961-RM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN‑ gamma (200 ng/mL) is neutralized (green line) by increasing concentrations of Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961). The ND50 is typically 1.5-5.0 µg/mL. |
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Related Product & Information
| Long Name | Interferon gamma |
|---|---|
| Entrez Gene IDs | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| Background | IFN-gamma |
| background_content | Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rhesus IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid sequence identity with human IFN-gamma, 57-66% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37-44% with cotton rat, mouse, and rat IFN‑ gamma. IFN‑ gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). |
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