抗IL-6抗体(Anti-IL-6, Porcine, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Porcine
Label	Unconjugated
Immunogen	E. coli-derived recombinant porcine IL-6Pro29-Met212Accession # P26893
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects porcine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human IL‑6, recombinant mouse IL‑6, and recombinant rat IL‑6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Porcine IL‑6 (Catalog # 686-PI)
Porcine IL-6 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Porcine IL‑6 Antibody (Catalog #AF686 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Porcine IL‑6 Biotinylated Antibody (Catalog #BAF686 )
ELISA Standard		Recombinant Porcine IL-6 Protein (Catalog #686-PI )
Neutralization	Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 12.5 ng/mL Recombinant Porcine IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody.Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

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References

Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
Jones, S.A. (2005) J. Immunol. 175:3468.
Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
Murakami, M. et al. (1993) Science 260:1808.
Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name:	Interleukin 6
Entrez Gene IDs:	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
Alternate Names:	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Related Research Areas

Show

Adipocytokines
Cancer Biomarkers
Conventional/Classical Dendritic Cells
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Cytokines and Receptors in Tfh Cell Differentiation and Function
Diabetes
gamma delta T Cells
Glucose Homeostasis
Helper T (Th) Cells
HIF Transcription Factors
IL-6 Family
Infectious Diseases
Inflammatory Mediators
Macrophage Activation Markers
Molecules Secreted by VSMC
Multiple Myeloma
Multiple Sclerosis and Myelin Related Molecules
Myeloid-derived Suppressor Cells (MDSC)
Neuroinflammation
Obesity
Plasmacytoid Dendritic Cells
T Cell Cytokine Signaling
Th17 Cells
Th22 Cells
Th9 Cells
Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Infect. Immun., 2017;85(4):. Species: Porcine Sample Type: Serum Application: ELISA Capture
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Porcine	Anonymous	11/08/2017			Image Details	ELISA: Porcine IL-6 Antibody [AF686].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Porcine
Other Experimental Details	The polyclonal antibody, AF696, was used as both the capture and detection antibody in a sandwich ELISA. It was specific and sensitive.

Neutralization

Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody. Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.

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Related Product & Information

Long Name	Interleukin 6
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
Background	IL-6
background_content	Background: IL-6 Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

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Citations

1. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
   Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
   Infect. Immun., 2017;85(4):.
   Species: Porcine
   Sample Type: Serum
   Application: ELISA Capture


2. TRAF6 is a novel NS3-interacting protein that inhibits classical swine fever virus replication
   Authors: H Lv, W Dong, Z Cao, X Li, J Wang, G Qian, Q Lv, C Wang, K Guo, Y Zhang
   Sci Rep, 2017;7(1):6737.
   Species: Porcine
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. Differential Effects of Escherichia coli Nissle and Lactobacillus rhamnosus Strain GG on Human Rotavirus Binding, Infection, and B Cell Immunity.
   Authors: Kandasamy S, Vlasova A, Fischer D, Kumar A, Chattha K, Rauf A, Shao L, Langel S, Rajashekara G, Saif L
   J Immunol, 2016;196(4):1780-9.
   Species: Porcine
   Sample Type: Whole Cells
   Application: Neut


4. Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
   Authors: Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH
   Eur. Heart J., 2008;29(12):1578-90.
   Species: Porcine
   Sample Type: Tissue Homogenates
   Application: WB


5. In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid.
   Authors: Saurer L, McCullough KC, Summerfield A
   J. Immunol., 2007;179(6):3504-14.
   Species: Porcine
   Sample Type: Whole Cells
   Application: Neut


6. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
   Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
   Intensive Care Med, 2007;33(6):1085-93.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


7. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
   Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
   J. Virol., 2006;80(1):372-82.
   Species: Porcine
   Sample Type: Serum
   Application: ELISA Development


8. Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
   Authors: Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
   Cardiovasc. Res., 2004;63(4):617-24.
   Species: Porcine
   Sample Type: Tissue Homogenates
   Application: WB


9. Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
   Authors: Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E
   Anesthesiology, 2004;100(4):861-70.
   Species: Porcine
   Sample Type: Plasma
   Application: ELISA Development

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