抗IL-4抗体(Anti-IL-4, Porcine, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：27191
IL-4に対する抗体(Anti-IL-4, Porcine, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年07月15日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-IL-4, Porcine, Goat-Poly |
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Anti-Porcine IL-4 Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年07月15日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IL-4, Porcine, Goat-Poly
文献数： 3
- 商品コード：AF654
- メーカー：RSD
- 包装：100μg
- 価格：¥81,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Porcine IL-4 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF654，スタンダードとして#654-P4-025を用いる。 別名：B cell growth factor 1 Genbank No： 3565 Protein Accession No： Q04745 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Porcine | 免疫動物 | Goat | ||||
| 交差性 | Porcine | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Porcine IL-4 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF654-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥25,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：B cell growth factor 1 Genbank No： 3565 Protein Accession No： Q04745 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Porcine | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Porcine |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant porcine IL-4His25-Cys133Accession # Q04745 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects porcine IL-4 in ELISAs and Western blots. In direct ELISAs, this antibody shows less than 10% cross-reactivity with recombinant mouse IL‑4 and recombinant rat IL‑4. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Porcine IL‑4 (Catalog # 654-P4) |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Porcine IL-4 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Porcine IL‑4 Antibody (Catalog #AF654 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Porcine IL‑4 Biotinylated Antibody (Catalog #BAF654 ) |
| ELISA Standard | Recombinant Porcine IL-4 Protein (Catalog #654-P4 ) | |
| Neutralization | Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 2 ng/mL Recombinant Porcine IL‑4. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization IL-4 Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL-4_AF654_Block_Neutralize_8940.jpg) click image to view larger | Cell Proliferation Induced by IL‑4 and Neutralization by Porcine IL‑4 Antibody.Recombinant Porcine IL‑4 (Catalog # 654-P4) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑4 (2 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND50 is typically 1‑5 µg/mL. |
| Immunocytochemistry |
![Immunocytochemistry IL-4 Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL-4_AF654_Immunocytochemistry_11366.jpg) click image to view larger | IL‑4 in Porcine PBMCs.IL‑4 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-4 | Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12). |
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References
| Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. | |
| Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1. | |
| Bailey, M. et al. (1993) Biochim. Biophys. Acta 1171:328. | |
| Redfield, C. et al. (1991) Biochemistry 30:11029. | |
| Stocker, C.J. et al. (2000) J. Immunol. 164:3309. | |
| Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237. | |
| Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701. | |
| Paludan, S.R. (1998) Scand. J. Immunol. 48:459. | |
| Corthay, A. (2006) Scand. J. Immunol. 64:93. | |
| Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. | |
| Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. | |
| Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303. | |
| Long Name: | Interleukin 4 |
| Entrez Gene IDs: | 3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit) |
| Alternate Names: | B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; Binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra |
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Related Research Areas
| Common gamma Chain Receptor Family | ||
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Angiogenesis | ||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
| Dendritic Cell Development | ||
| gamma delta T Cells | ||
| Group 2 ILCs | ||
| Inflammatory Mediators | ||
| Multiple Sclerosis and Myelin Related Molecules | ||
| Natural Killer T (NKT) Cells | ||
| Regulatory T Cells (Tregs) | ||
| T Cell Cytokine Signaling | ||
| Th2 Cells | ||
| Th9 Cells | ||
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| Neutralization | |
|---|---|
 | Cell Proliferation Induced by IL‑4 and Neutralization by Porcine IL‑4 Antibody. Recombinant Porcine IL‑4 (Catalog # 654-P4) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑4 (2 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND50 is typically 1‑5 µg/mL. |
| Immunocytochemistry | |
 | IL‑4 in Porcine PBMCs. IL‑4 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Long Name | Interleukin 4 |
|---|---|
| Entrez Gene IDs | 3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit) |
| Background | IL-4 |
| background_content | Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
Infect. Immun., 2017;85(4):.
Species: Porcine
Sample Type: Serum
Application: ELISA Capture - Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Authors: Nadworny PL, Wang J, Tredget EE
J Inflamm (Lond), 2010;7(0):13.
Species: Porcine
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
J. Virol., 2006;80(1):372-82.
Species: Porcine
Sample Type: Whole Cells
Application: ELISpot Development
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