抗IFN-γ抗体(Anti-IFN-γ, Cotton Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Cotton Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant cotton rat IFN-gamma His21-Ile170Accession # Q9QXX2
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects cotton rat IFN‑ gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant canine IFN-gamma, recombinant feline IFN-gamma, and recombinant human IFN-gamma, recombinant mouse IFN-gamma, recombinant rat IFN-gamma, and recombinant porcine IFN-gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG)
Cotton Rat IFN-gamma Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Cotton Rat IFN‑ gamma Antibody (Catalog #AF565 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Cotton Rat IFN‑ gamma Biotinylated Antibody (Catalog #BAF565 )
ELISA Standard		Recombinant Cotton Rat IFN-gamma Protein (Catalog #565-RG )
Neutralization	Measured by its ability to neutralize IFN‑ gamma inhibition of EMC-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 20 ng/mL Recombinant Cotton Rat IFN‑ gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	IFN‑ gamma  Inhibition of EMC-induced Cytopathy and Neutralization by Cotton Rat IFN‑ gamma  Antibody. Recombinant Cotton Rat IFN‑ gamma (Catalog # 565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN‑ gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND50 is typically 0.3‑1.2 µg/mL.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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References

Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
Pestka, S. et al. (2004) Immunol. Rev. 202:8.
Houard, S. et al. (1999) Gene 240:107.
Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name:	Interferon gamma
Entrez Gene IDs:	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:	IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Related Research Areas

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

Group 1 ILCs

IL-10/Interferon Family

Infectious Diseases

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Macrophage Activation Markers

Molecules Secreted by VSMC

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

Natural Killer T (NKT) Cells

NCR+ILC3

NCR-ILC3

Neuroinflammation

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th1 Cells

Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection Authors: MC Patel, LM Pletneva, MS Boukhvalov, SN Vogel, AE Kajon, JCG Blanco Front Microbiol, 2017;8(0):1646. Species: Cotton Rat Sample Type: Whole Cells Application: ELISA Capture

Neutralization

IFN‑ gamma  Inhibition of EMC-induced Cytopathy and Neutralization by Cotton Rat IFN‑ gamma  Antibody. Recombinant Cotton Rat IFN‑ gamma (Catalog # 565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN‑ gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND50 is typically 0.3‑1.2 µg/mL.

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Related Product & Information

Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Background	IFN-gamma
background_content	Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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Citations

1. Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection
   Authors: MC Patel, LM Pletneva, MS Boukhvalov, SN Vogel, AE Kajon, JCG Blanco
   Front Microbiol, 2017;8(0):1646.
   Species: Cotton Rat
   Sample Type: Whole Cells
   Application: ELISA Capture


2. Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats
   PLoS ONE, 2016;11(5):e0155777.
   Species: Cotton Rat
   Sample Type: Protein
   Application: Flow


3. Role of AKT kinase in measles virus replication.
   Authors: Carsillo M, Kim D, Niewiesk S
   J. Virol., 2010;84(4):2180-3.
   Species: Cotton Rat
   Sample Type: Whole Cells
   Application: ELISpot Development


4. Sendai virus recombinant vaccine expressing hPIV-3 HN or F elicits protective immunity and combines with a second recombinant to prevent hPIV-1, hPIV-3 and RSV infections.
   Authors: Zhan X, Slobod KS, Krishnamurthy S, Luque LE, Takimoto T, Jones B, Surman S, Russell CJ, Portner A, Hurwitz JL
   Vaccine, 2008;26(27):3480-8.
   Species: Cotton Rat
   Sample Type: Peptide
   Application: ELISpot Development


5. Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B.
   Authors: Zhan X, Hurwitz JL, Krishnamurthy S, Takimoto T, Boyd K, Scroggs RA, Surman S, Portner A, Slobod KS
   Vaccine, 2007;25(52):8782-93.
   Species: Cotton Rat
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. Developmental pattern of expression of BMP receptors and Smads and activation of Smad1 and Smad5 by BMP9 in mouse basal forebrain.
   Authors: Lopez-Coviella I, Mellott TM, Kovacheva VP, Berse B, Slack BE, Zemelko V, Schnitzler A, Blusztajn JK
   Brain Res., 2006;1088(1):49-56.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP

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