抗IL-10抗体(Anti-IL-10, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat IL‑10Ser19-Asn178Accession # P29456
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse, rat, and cotton rat IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-10, recombinant guinea pig IL-10, recombinant viral IL-10 and recombinant canine IL-10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	Immersion fixed rat splenocytes
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 40 ng/mL Recombinant Rat IL‑10.

Western Blot
	Detection of Recombinant Mouse and Rat IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑10 (Catalog # 417-ML), Recombinant Human IL‑10 (Catalog # 217-IL), Recombinant Rat IL‑10 (Catalog # 522-RLB), and Recombinant Human IL‑26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	Cell Proliferation Induced by IL‑10 and Neutralization by Rat IL‑10 Antibody. Recombinant Rat IL‑10 (Catalog # 522-RL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND50 is typically 0.3-1.0 µg/mL.

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Related Product & Information

Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Background	IL-10
background_content	Background: IL-10 Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL‑22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%‑79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

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Citations

1. Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization
   Authors: M Viladomiu, J Bassaganya, N Tubau-Juni, B Kronsteine, A Leber, CW Philipson, V Zoccoli-Ro, R Hontecilla
   J. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


2. ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
   Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
   J. Neurosci., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion
   Authors: L Cambier, G de Couto, A Ibrahim, AK Echavez, J Valle, W Liu, M Kreke, RR Smith, L Marbán, E Marbán
   EMBO Mol Med, 2017;9(3):337-352.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


4. A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
   Authors: JE Lim, E Chung, Y Son
   Sci Rep, 2017;7(1):9417.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


5. Autocrine interleukin-10 mediates glucagon-like peptide-1 receptor-induced spinal microglial ?-endorphin expression
   Authors: HY Wu, XQ Tang, XF Mao, YX Wang
   J. Neurosci., 2017;0(0):.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC-Fr


6. Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10.
   Authors: Xie L, Choudhury G, Winters A, Yang S, Jin K
   Eur J Immunol, 2015;45(1):180-91.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


7. Immunization with a Myelin-Derived Antigen Activates the Brain's Choroid Plexus for Recruitment of Immunoregulatory Cells to the CNS and Attenuates Disease Progression in a Mouse Model of ALS.
   Authors: Kunis G, Baruch K, Miller O, Schwartz M
   J Neurosci, 2015;35(16):6381-93.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. CNS repair requires both effector and regulatory T cells with distinct temporal and spatial profiles.
   Authors: Raposo C, Graubardt N, Cohen M, Eitan C, London A, Berkutzki T, Schwartz M
   J Neurosci, 2014;34(31):10141-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
   Authors: Furuhashi, Kazuhiro, Tsuboi, Naotake, Shimizu, Asuka, Katsuno, Takayuki, Kim, Hangsoo, Saka, Yosuke, Ozaki, Takenori, Sado, Yoshikaz, Imai, Enyu, Matsuo, Seiichi, Maruyama, Shoichi
   J Am Soc Nephrol, 2013;24(4):587-603.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


10. Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.
    Authors: Holladay C, Power K, Sefton M, O'Brien T, Gallagher WM, Pandit A
    Mol. Ther., 2011;19(5):969-78.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen

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