抗IL-10抗体(Anti-IL-10, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL‑10Ser19-Ser178Accession # NP_034678
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects mouse IL-10 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL‑10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL‑10 (Catalog # 417-ML)
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line [Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507]. The Neutralization Dose (ND50) is typically 2-4 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.

Neutralization

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑10 Polyclonal Antibody (Catalog # AB-417-NA). The ND50 is typically 2-4 µg/mL.

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Related Product & Information

Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Background	IL-10
background_content	Background: IL-10 Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%‑77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

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Citations

1. Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
   Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N
   J Thromb Haemost, 2014;12(7):1156-65.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


2. Low-Dose Lipopolysaccharide Pretreatment Suppresses Choroidal Neovascularization via IL-10 Induction.
   Authors: Matsumura N, Kamei M, Tsujikawa M
   PLoS ONE, 2012;7(7):e39890.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


3. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
   Authors: Wang H, Cheng F
   J. Immunol., 2011;186(0):3986.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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