抗TGF-β Receptor Ⅱ抗体(Anti-TGF-β Receptor Ⅱ, Human, Goat-Poly antibody)

掲載日情報:2021/01/28 現在Webページ番号:26850

TGF-β Receptor Ⅱに対する抗体(Anti-TGF-β Receptor Ⅱ, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年02月16日 00時00分現在]

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納期 文献数
Anti-TGF-β Receptor II, Human, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 33
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説明文
マッチドペア:Human TGF-β RII サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF241,スタンダードとして#241-R2-025を用いる。
別名:AAT3
Genbank No: 7048
Protein Accession No: P37173.2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,FCM,IHC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
Helper T Cells Marker Antibodies
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
Anti-Human TGF-beta RII Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
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説明文
※受注発注品。形状:溶液または凍結乾燥
別名:AAT3
Genbank No: 7048
Protein Accession No: P37173.2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,FCM,IHC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
Helper T Cells Marker Antibodies
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2025年02月16日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-TGF-β Receptor II, Human, Goat-Poly

文献数: 33

説明文 マッチドペア:Human TGF-β RII サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF241,スタンダードとして#241-R2-025を用いる。
別名:AAT3
Genbank No: 7048
Protein Accession No: P37173.2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ELISA,FCM,IHC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
Helper T Cells Marker Antibodies
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

Anti-Human TGF-beta RII Affinity Purified Polyclonal Ab

文献数: 2

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:AAT3
Genbank No: 7048
Protein Accession No: P37173.2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 ELISA,FCM,IHC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
Helper T Cells Marker Antibodies
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenMouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RIIIle24-Asp159Accession # P37173.2
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouseTGF‑ beta  RII is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot2 µg/mLSee below
Flow Cytometry1 µg/106 cellsSee below
Immunohistochemistry5-15 µg/mLSee below
CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Human TGF-beta RII Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)0.2-0.8 µg/mL Human TGF‑ beta  RII Antibody (Catalog #AF-241-NA )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Human TGF‑ beta  RII Biotinylated Antibody (Catalog #BAF241 )
ELISA Standard  Recombinant Human TGF-beta RII Protein (Catalog #241-R2 )
NeutralizationMeasured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the TF‑1 human erythroleukemic cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 5‑20 µg/mL in the presence of 0.04 ng/mL Recombinant Human TGF‑ beta 1 and 5 ng/mL Recombinant Human IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot Western Blot TGF-beta RII Antibody [Unconjugated]
click image to view larger
Detection of Human TGF‑ beta  RII by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF‑ beta  RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry Flow Cytometry TGF-beta RII Antibody [Unconjugated]
click image to view larger
Detection of TGF-beta  RII in Human Lymphocytes by Flow Cytometry.Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta  RII Antigen Affinity-purified Poly­clonal Antibody (Catalog #
AF‑241‑NA, filled histogram) or control antibody (Catalog #
AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunohistochemistry Immunohistochemistry TGF-beta RII Antibody [Unconjugated]
click image to view larger
TGF-beta  RII in Human Pituitary.TGF-beta  RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization Neutralization TGF-beta RII Antibody [Unconjugated]
click image to view larger
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta  RII Antibody.Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑4 (5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.04 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti- Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically
10‑20 µg/mL.
ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RII

TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.

Long Name:Transforming Growth Factor beta Receptor II
Entrez Gene IDs:7048 (Human); 21813 (Mouse)
Alternate Names:AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

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Related Research Areas

Airway Inflammation and Remodeling
Cytokine and Growth Factor Receptors on VSMC
Cytokines and Receptors in Angiogenesis
Preeclampsia
Regulatory T Cells (Tregs)
T Cell Cytokine Signaling
TGF-beta Family
TGF-beta Superfamily
Th17 Cells
Th22 Cells
Th9 Cells
  1. TGF-? contamination of purified recombinant GDF15
    Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
    PLoS ONE, 2017;12(11):e0187349.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut


Western Blot
Detection of Human TGF‑ beta  RII by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF‑ beta  RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
Detection of TGF-beta  RII in Human Lymphocytes by Flow Cytometry.
Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta  RII Antigen Affinity-purified Poly­clonal Antibody (Catalog #AF‑241‑NA, filled histogram) or control antibody (Catalog #AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunohistochemistry
TGF-beta  RII in Human Pituitary.
TGF-beta  RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta  RII Antibody.
Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑4 (5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.04 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti- Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically10‑20 µg/mL.


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Related Product & Information

Long NameTransforming Growth Factor beta Receptor II
Entrez Gene IDs7048 (Human); 21813 (Mouse)
BackgroundTGF-beta RII
background_contentBackground:
TGF-beta RII
TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
  1. TGF-? contamination of purified recombinant GDF15
    Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
    PLoS ONE, 2017;12(11):e0187349.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

  2. 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats
    PLoS ONE, 2016;11(8):e0161782.
    Species: Rat
    Sample Type: Whole Cells
    Application: Blocking

  3. Clathrin light chains' role in selective endocytosis influences antibody isotype switching
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

  4. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of alphavbeta6 integrin in DCIS identifies high-risk patients and predicts recurrence.
    Authors: Allen M, Thomas G, Clark S, Dawoud M, Vallath S, Payne S, Gomm J, Dreger S, Dickinson S, Edwards D, Pennington C, Sestak I, Cuzick J, Marshall J, Hart I, Jones J
    Clin Cancer Res, 2014;20(2):344-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut

  5. CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism.
    Authors: Miller M, Petty C, Tompkins M, Fogle J
    Virol J, 2014;11(0):7.
    Species: Feline
    Sample Type: Whole Cells
    Application: Neut

  6. Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.
    Authors: Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A
    J Clin Invest, 2014;124(8):3295-310.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Neut

  7. T-cell TGF-beta signaling abrogation restricts medulloblastoma progression.
    Authors: Gate D, Danielpour M, Rodriguez J, Kim G, Levy R, Bannykh S, Breunig J, Kaech S, Flavell R, Town T
    Proc Natl Acad Sci U S A, 2014;111(33):E3458-66.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC

  8. Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion.
    Authors: Kenny H, Chiang C, White E, Schryver E, Habis M, Romero I, Ladanyi A, Penicka C, George J, Matlin K, Montag A, Wroblewski K, Yamada S, Mazar A, Bowtell D, Lengyel E
    J Clin Invest, 2014;124(10):4614-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

  9. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
    Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
    Cancer Res, 2013;73(7):2333-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

  10. Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
    Authors: Rai D, Kim SW, McKeller MR
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut



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