抗TGF-β Receptor Ⅱ抗体(Anti-TGF-β Receptor Ⅱ, Human, Goat-Poly antibody)
掲載日情報:2021/01/28 現在Webページ番号:26850
TGF-β Receptor Ⅱに対する抗体(Anti-TGF-β Receptor Ⅱ, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年02月16日 00時00分現在]
詳細 | 商品名 |
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Anti-TGF-β Receptor II, Human, Goat-Poly |
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Anti-Human TGF-beta RII Affinity Purified Polyclonal Ab |
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[在庫・価格 :2025年02月16日 00時00分現在]
Anti-TGF-β Receptor II, Human, Goat-Poly
文献数: 33
- 商品コード:AF-241-NA
- メーカー:RSD
- 包装:250μg
- 価格:¥112,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Human TGF-β RII サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF241,スタンダードとして#241-R2-025を用いる。 別名:AAT3 Genbank No: 7048 Protein Accession No: P37173.2 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Goat | ||||
交差性 | Human | 適用 | ELISA,FCM,IHC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Helper T Cells Marker Antibodies |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Human TGF-beta RII Affinity Purified Polyclonal Ab
文献数: 2
- 商品コード:AF-241-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:AAT3 Genbank No: 7048 Protein Accession No: P37173.2 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Goat | |||||
交差性 | Human | 適用 | ELISA,FCM,IHC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Helper T Cells Marker Antibodies 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Human |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RIIIle24-Asp159Accession # P37173.2 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects human TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouseTGF‑ beta RII is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 2 µg/mL | See below |
Flow Cytometry | 1 µg/106 cells | See below |
Immunohistochemistry | 5-15 µg/mL | See below |
CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
Human TGF-beta RII Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Human TGF‑ beta RII Antibody (Catalog #AF-241-NA ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human TGF‑ beta RII Biotinylated Antibody (Catalog #BAF241 ) |
ELISA Standard | Recombinant Human TGF-beta RII Protein (Catalog #241-R2 ) | |
Neutralization | Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the TF‑1 human erythroleukemic cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 5‑20 µg/mL in the presence of 0.04 ng/mL Recombinant Human TGF‑ beta 1 and 5 ng/mL Recombinant Human IL‑4. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Western Blot |
![]() click image to view larger | Detection of Human TGF‑ beta RII by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF‑ beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF‑ beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
Flow Cytometry |
![]() click image to view larger | Detection of TGF-beta RII in Human Lymphocytes by Flow Cytometry.Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑241‑NA, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). |
Immunohistochemistry |
![]() click image to view larger | TGF-beta RII in Human Pituitary.TGF-beta RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
Neutralization |
![]() click image to view larger | TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta RII Antibody.Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑4 (5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.04 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti- Human TGF‑ beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically 10‑20 µg/mL. |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: TGF-beta RII | TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described. |
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Long Name: | Transforming Growth Factor beta Receptor II | |
Entrez Gene IDs: | 7048 (Human); 21813 (Mouse) | |
Alternate Names: | AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II |
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Related Research Areas
Airway Inflammation and Remodeling | ||
Cytokine and Growth Factor Receptors on VSMC | ||
Cytokines and Receptors in Angiogenesis | ||
Preeclampsia | ||
Regulatory T Cells (Tregs) | ||
T Cell Cytokine Signaling | ||
TGF-beta Family | ||
TGF-beta Superfamily | ||
Th17 Cells | ||
Th22 Cells | ||
Th9 Cells | ||
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Related Product & Information
Long Name | Transforming Growth Factor beta Receptor II |
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Entrez Gene IDs | 7048 (Human); 21813 (Mouse) |
Background | TGF-beta RII |
background_content | Background: TGF-beta RII TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described. |
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Citations
- TGF-? contamination of purified recombinant GDF15
Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
PLoS ONE, 2017;12(11):e0187349.
Species: Human
Sample Type: Whole Cells
Application: Neut - 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats
PLoS ONE, 2016;11(8):e0161782.
Species: Rat
Sample Type: Whole Cells
Application: Blocking - Clathrin light chains' role in selective endocytosis influences antibody isotype switching
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay - Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of alphavbeta6 integrin in DCIS identifies high-risk patients and predicts recurrence.
Authors: Allen M, Thomas G, Clark S, Dawoud M, Vallath S, Payne S, Gomm J, Dreger S, Dickinson S, Edwards D, Pennington C, Sestak I, Cuzick J, Marshall J, Hart I, Jones J
Clin Cancer Res, 2014;20(2):344-57.
Species: Mouse
Sample Type: Whole Cells
Application: Neut - CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism.
Authors: Miller M, Petty C, Tompkins M, Fogle J
Virol J, 2014;11(0):7.
Species: Feline
Sample Type: Whole Cells
Application: Neut - Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling.
Authors: Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A
J Clin Invest, 2014;124(8):3295-310.
Species: Mouse
Sample Type: Cell Lysates
Application: Neut - T-cell TGF-beta signaling abrogation restricts medulloblastoma progression.
Authors: Gate D, Danielpour M, Rodriguez J, Kim G, Levy R, Bannykh S, Breunig J, Kaech S, Flavell R, Town T
Proc Natl Acad Sci U S A, 2014;111(33):E3458-66.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion.
Authors: Kenny H, Chiang C, White E, Schryver E, Habis M, Romero I, Ladanyi A, Penicka C, George J, Matlin K, Montag A, Wroblewski K, Yamada S, Mazar A, Bowtell D, Lengyel E
J Clin Invest, 2014;124(10):4614-28.
Species: Human
Sample Type: Whole Cells
Application: Neut - TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
Cancer Res, 2013;73(7):2333-44.
Species: Human
Sample Type: Cell Lysates
Application: WB - Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
Authors: Rai D, Kim SW, McKeller MR
Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6.
Species: Human
Sample Type: Whole Cells
Application: Neut
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