抗IL-10抗体(Anti-IL-10, HCMV, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Viral
Label	Unconjugated
Immunogen	E. coli-derived recombinant viral HCMV IL‑10Ala26-Lys176Accession # AAF63437
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects viral HCMV IL-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑10, recombinant canine IL‑10, recombinant feline IL‑10, recombinant cotton rat IL‑10, recombinant rat IL‑10, recombinant porcine IL‑10, recombinant human IL‑10, and recombinant Epstein-Barr virus encoded viral IL‑10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Viral HCMV IL‑10 (Catalog # 117-VL)
Neutralization	Measured by its ability to neutralize IL‑10-mediated inhibition of LPS-induced IL-1 beta secretion in human peripheral blood lymphocytes (PBL). The Neutralization Dose (ND50) is typically 0.15-0.9 μg/mL in the presence of 2 ng/mL Recombinant Viral HCMV IL‑10.

Neutralization

IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody. Recombinant Viral HCMV IL‑10 (Catalog # 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND50 is typically 0.15-0.9 μg/mL.

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Related Product & Information

Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Background	IL-10
background_content	Background: IL-10 Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

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Citations

1. Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
   Authors: J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina
   Sci Rep, 2017;7(0):39710.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


2. cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells.
   Authors: Valle Oseguera, Cendy A, Spencer, Juliet V
   PLoS ONE, 2014;9(2):e88708.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
   Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
   BMC Biotechnol, 2013;13(0):82.
   Species: Virus
   Sample Type: Cell Fraction
   Application: ELISA Development


4. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
   Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
   J. Virol., 2008;82(7):3736-50.
   Species: Virus
   Sample Type: Recombinant Protein
   Application: WB


5. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
   Authors: Chang WL, Baumgarth N, Yu D, Barry PA
   J. Virol., 2004;78(16):8720-31.
   Species: Virus
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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