抗TGF-beta 1/1.2抗体(Anti-TGF-β1, Human, Chicken-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26753

TGF-beta 1/1.2に対する抗体(Anti-TGF-β1, Human, Chicken-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-TGF-β1, Human, Chicken-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 8
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説明文
別名:TGF-beta 1
Protein Accession No: P01137
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Chicken クラス IgY 標識 Unlabeled
交差性 Multi-species 適用 Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
Myeloid-derived Suppressor Cell Marker
関連記事
Anti-TGF-β1, Human, Chicken-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 8
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
別名:TGF-beta 1
Protein Accession No: P01137
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Chicken クラス IgY 標識 Unlabeled
交差性 Multi-species 適用 Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
Myeloid-derived Suppressor Cell Marker
関連記事

[在庫・価格 :2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-TGF-β1, Human, Chicken-Poly

文献数: 8

説明文 別名:TGF-beta 1
Protein Accession No: P01137
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Chicken
交差性 Multi-species 適用 Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgY
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
Myeloid-derived Suppressor Cell Marker
関連記事

Anti-TGF-β1, Human, Chicken-Poly

文献数: 8

説明文 別名:TGF-beta 1
Protein Accession No: P01137
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Chicken
交差性 Multi-species 適用 Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgY
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
Myeloid-derived Suppressor Cell Marker
関連記事



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Product Details

LabelUnconjugated
ImmunogenChinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 (R&D Systems, Catalog # 240-B)Ala279-Ser390Accession # P01137
SourcePolyclonal Chicken IgY
PurificationIon exchange-purified from egg yolks
SpecificityDetects TGF‑ beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant amphibian TGF‑ beta 5 is observed and less than 2% cross-reactivity with recombinant human TGF‑ beta 3, recombinant chicken TGF‑ beta 3, and porcine TGF‑ beta 2 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Human TGF-beta 1 (Catalog # 240-B)
Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)
NeutralizationMeasured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 0.25 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.


Neutralization
TGF‑ beta 1 Inhibition ofIL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1/1.2 Antibody.
Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF‑ beta 1/1.2 Polyclonal Antibody (Catalog # AB-101-NA). The ND50 is typically 0.2-0.6 µg/mL.


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Related Product & Information

Long NameTransforming Growth Factor beta 1 and 1.2
Entrez Gene IDs7040 (Human); 21803 (Mouse); 59086 (Rat)
BackgroundTGF-beta 1/1.2
background_contentBackground:
TGF-beta 1/1.2
TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Cancer associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-�-dependent pathways in genotype-specific oral cancer
    Authors: S S Prime
    Carcinogenesis, 2016;0(0):.
    Species: Human
    Sample Type: Conditioned Media
    Application: Neut

  2. Cord blood mesenchymal stem cells suppress DC-T Cell proliferation via prostaglandin B2.
    Authors: van den Berk L, Jansen B, Snowden S, Siebers-Vermeulen K, Gilissen C, Kogler G, Figdor C, Wheelock C, Torensma R
    Stem Cells Dev, 2014;23(14):1582-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

  3. Isoform specificity of commercially-available anti-TGF-beta antibodies.
    Authors: Mozes MM, Hodics T, Kopp JB
    J. Immunol. Methods, 1999;225(1):87-93.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB



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