Mouse IFN-beta Quantikine ELISA Kit
掲載日情報：2018/02/01 現在Webページ番号：206230
R&D Systems製のMouse IFN-betaを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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[在庫・価格 ：2025年04月27日 00時00分現在]
Mouse IFN-beta Quantikine ELISA Kit
文献数： 7
- 商品コード：MIFNB0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：1.89 pg/mL，測定範囲：15.6-1,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Mouse，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Sandwich，アッセイ数：96，測定時間：4.5 hours，測定因子：IFN-beta 別名：Fibroblast interferon Genbank No： 3456 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Human/Mouse IFN-β ELISA Kit Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
- Sensitivity
- 1.89 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
- Specificity
- Natural and recombinant mouse IFN-beta.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine® Mouse IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-beta in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse IFN-beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IFN-beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-beta.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Recovery
The recovery of mouse IFN-beta spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 88-106 |
| EDTA Plasma (n=4) | 99 | 89-106 |
| Heparin Plasma (n=4) | 94 | 83-100 |
| Serum (n=4) | 94 | 89-97 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Entrez Gene IDs
- 3456 (Human); 15977 (Mouse); 24481 (Rat)
- Background
- IFN-beta
- Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
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