Human Serum TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
掲載日情報：2018/02/01 現在Webページ番号：206221
R&D Systems製のHuman Serum TIM-1/KIM-1/HAVCRを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月20日 18時15分現在]
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Human Serum TIM-1/KIM-1/HAVCR Quantikine ELISA Kit |
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[在庫・価格 ：2024年04月20日 18時15分現在]
Human Serum TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
文献数： 3
- 商品コード：DSKM100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：3.63 pg/mL，測定範囲：10.90 - 700 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Sandwich，アッセイ数：96，別売品コントロール：QC229，測定時間：4.5 hours，測定因子：TIM-1/KIM-1/HAVCR 別名：CD365 Genbank No： 26762 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Immune Checkpoint Protein ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
- Sensitivity
- 3.63 pg/mL
- Assay Range
- 10.9 - 700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
- Specificity
- Natural and recombinant human TIM-1.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine® Human Serum TIM-1/KIM-1/HAVCR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human TIM-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human TIM-1 and antibodies raised against the recombinant factor. Natural human TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative levels of natural human TIM-1.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Recovery
The recovery of human TIM-1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 94 | 89-102 |
| EDTA Plasma (n=4) | 93 | 80-108 |
| Heparin Plasma (n=4) | 91 | 81-104 |
| Serum (n=4) | 94 | 82-109 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TIM-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Entrez Gene IDs
- 26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
- Background
- TIM-1/KIM-1/HAVCR
- TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.
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