Human Total IL-18/IL-1F4 Quantikine ELISA Kit
掲載日情報：2018/02/01 現在Webページ番号：206220
R&D Systems製のHuman Total IL-18/IL-1F4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Human Total IL-18/IL-1F4 Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Human Total IL-18/IL-1F4 Quantikine ELISA Kit
文献数： 8
- 商品コード：DL180
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：5.15 pg/mL，測定範囲：15.6-1,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Saliva，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Sandwich，アッセイ数：96，別売品コントロール：QC231，測定時間：4.0 hours，測定因子：Total IL-18, IL-1F4 別名：Iboctadekin Genbank No： 3606 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Human Total IL-18/IL-1F4 Quantikine ELISA Kit Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Length
- 4.0 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (25 uL), Urine (50 uL)
- Sensitivity
- 5.15 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
- Specificity
- Natural and recombinant human Total IL-18.
- Cross-reactivity
- Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Urine
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Recovery
The recovery of human Total IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 90-102 |
| EDTA Plasma (n=4) | 104 | 93-113 |
| Heparin Plasma (n=4) | 102 | 88-110 |
| Saliva (n=4) | 108 | 94-120 |
| Serum (n=4) | 100 | 96-105 |
| Urine (n=4) | 91 | 84-100 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total IL-18 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Entrez Gene IDs
- 3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
- Background
- IL-18/IL-1F4
- Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-γ and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-γ that inhibits IgE production.
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