Human IFN-beta Quantikine ELISA Kit
掲載日情報:2018/02/01 現在Webページ番号:206218
R&D Systems製のHuman IFN-betaを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2025年04月27日 00時00分現在]
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[在庫・価格 :2025年04月27日 00時00分現在]
Human IFN-beta Quantikine ELISA Kit
文献数: 8
- 商品コード:DIFNB0
- メーカー:RSD
- 包装:1kit
- 価格:¥116,000
- 在庫:2個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 感度:0.781 pg/mL,測定範囲:7.81-500 pg/mL,測定試料:Cell Culture Supernatant,種交差性:Human,検出方法:colorimetric,測定波長:450 nm,アッセイ法:Sandwich,アッセイ数:96,別売品コントロール:QC243,測定時間:4.5 hours,測定因子:IFN-beta 別名:Fibroblast interferon Genbank No: 3456 |
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保存条件 | 4℃ | 法規備考 | |
掲載カタログ |
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製品記事 | Quantikine Human/Mouse IFN-β ELISA Kit Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ(R&D Systems) |
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関連記事 | R&D Systems Quantikine ELISA Kit/DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL)
- Sensitivity
- 0.781 pg/mL
- Assay Range
- 7.8 - 500 pg/mL (Cell Culture Supernates)
- Specificity
- Natural and recombinant human IFN-beta.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine® Human IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-beta in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates
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Recovery
The recovery of human IFN-beta spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
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Cell Culture Supernates (n=5) | 107 | 95-111 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Entrez Gene IDs
- 3456 (Human); 15977 (Mouse); 24481 (Rat)
- Background
- IFN-beta
- Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
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