Human Erythropoietin Quantikine ELISA Kit, RUO
掲載日情報:2018/02/01 現在Webページ番号:206213
R&D Systems製のHuman Erythropoietin, RUOを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2025年05月01日 10時55分現在]
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Human Erythropoietin Quantikine ELISA Kit, RUO |
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[在庫・価格 :2025年05月01日 10時55分現在]
Human Erythropoietin Quantikine ELISA Kit, RUO
文献数: 1
- 商品コード:DEPRU0
- メーカー:RSD
- 包装:1kit
- 価格:¥154,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 感度:0.6 mIU/mL,測定範囲:2.5-200 mIU/mL,測定試料:Serum, Plasma,種交差性:Human,検出方法:colorimetric,測定波長:450 nm,アッセイ法:Sandwich,アッセイ数:96,測定時間:4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol),測定因子:Erythropoietin, EPO 別名:EP Genbank No: 2056 |
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保存条件 | 4℃ | 法規備考 | |
掲載カタログ |
ニュース2019年12月15日号 p.7
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製品記事 | HIF(Hypoxia Inducible Factor、低酸素誘導因子)関連製品 R&D Systems社 Human Epo(Erythropoietin)研究用製品 Human Erythropoietin (Epo) Quantikine ELISA Kit ELISA Kit製品に関するFAQ(R&D Systems) 日本語マニュアル配布中! |
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関連記事 | R&D Systems Quantikine ELISA Kit/DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Length
- 4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
- Sample Type & Volume Required Per Well
- Serum (100 uL), EDTA Plasma (100 uL)
- Specificity
- Natural and recombinant human Epo.
- Interference
- Interference observed with other substances.
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Product Summary
The Quantikine® Human Epo ELISA uses a monoclonal antibody and polyclonal antibody conjugate in a sandwich ELISA format. The assay is designed to measure Epo levels in serum or EDTA plasma in less than 4.5 hours, or less than 2.5 hours using the shaker protocol.
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Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested thirty times to assess intra-assay precision Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components.
Benchtop Protocol | Shaker Protocol | ||||||||
Sample |
1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
Mean |
5.82 | 16.01 | 25.18 | 79.02 | 6.45 | 16.22 | 26.35 | 84.14 | |
Within Assay Precision (%CV) |
4.95 | 2.84 | 5.22 | 3.06 | 7.82 | 3.02 | 2.18 | 2.42 | |
Total Precision (%CV) |
8.25 | 4.3 | 5.9 | 4.24 | 10.3 | 3.74 | 4.2 | 3.71 |
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Recovery
The recovery of human Epo spiked to levels throughout the range of the assay was evaluated.
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Linearity
Five matched serum and plasma specimens containing elevated Epo concentrations were diluted with Specimen Diluent and assayed using the Quantikine Human Epo ELISA. Diluted specimens demonstrated very good linearity when compared to neat concentrations of Epo.
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Additional Infomation
- Entrez Gene IDs
- 2056 (Human); 13856 (Mouse); 24335 (Rat)
- Background
- Erythropoietin
- Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level. The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
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