抗Guinea Pig TNF-alpha抗体(Anti-Guinea Pig TNF-alpha antibody)
掲載日情報:2021/01/28 現在Webページ番号:199460
Guinea Pig TNF-alphaに対する抗体(Anti-Guinea Pig TNF-alpha )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 :2025年04月26日 20時55分現在]
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Anti-Guinea Pig TNF-alpha MAb (Clone 569826) |
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[在庫・価格 :2025年04月26日 20時55分現在]
Anti-Guinea Pig TNF-alpha MAb (Clone 569826)
文献数: 0
- 商品コード:MAB5035
- メーカー:RSD
- 包装:500μg
- 価格:¥121,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Guinea Pig TNF-α サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAM50351,スタンダードとして#5035-TG-025を用いる。 別名:APC1 protein クローン:569826 Genbank No: 7124 Protein Accession No: P51435 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Guinea Pig | 適用 | ELISA | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) Helper T Cells Marker Antibodies |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Guinea Pig TNF-alpha MAb (Clone 569826)
文献数: 0
- 商品コード:MAB5035-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:APC1 protein クローン:569826 Genbank No: 7124 Protein Accession No: P51435 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Guinea Pig | 適用 | ELISA | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) Helper T Cells Marker Antibodies 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Guinea Pig |
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Label | Unconjugated |
Immunogen | E. coli-derived recombinant guinea pig TNF-alpha Leu79-Leu234Accession # P51435 |
Source | Monoclonal Mouse IgG1 Clone # 569826 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects guinea pig TNF‑ alpha in ELISAs. In sandwich immunoassays, no cross-reactivity with human, mouse, rat, porcine, canine, feline, equine, bovine, or cotton rat TNF-alpha is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Guinea Pig TNF-alpha Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Guinea Pig TNF‑ alpha Antibody (Catalog #MAB5035 ) |
ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Guinea Pig TNF‑ alpha Biotinylated Antibody (Catalog #BAM50351 ) |
ELISA Standard | Recombinant Guinea Pig TNF-alpha Protein (Catalog #5035-TG ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: TNF-alpha | Tumornecrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, isthe prototypic ligand of the TNF superfamily. It is a pleiotropicmolecule that plays a central role in inflammation, apoptosis, andimmune system development. TNF-alpha is produced by a wide variety of immuneand epithelial cell types (1, 2). Guinea pig TNF-alpha consists of a 35amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a155 aa extracellular domain (ECD) (3). Within the ECD, guinea pig TNF-alphashares 80% - 83% aa sequence identity with human, mouse, and ratTNF-alpha. The 26 kDa type 2 transmembrane protein is assembledintracellularly to form a noncovalently linked homotrimer (4). Ligationof this complex induces reverse signaling that promotes lymphocyteco-stimulation but diminishes monocyte responsiveness (5). Cleavage ofmembrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimericform of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and thehematopoietic cell-restricted TNF RII, both of which are also expressedas homotrimers (1, 8). TNF-alpha regulates lymphoid tissue developmentthrough control of apoptosis (2). It also promotes inflammatoryresponses by inducing the activation of vascular endothelial cells andmacrophages (2). TNF-alpha is a key cytokine in the development of severalinflammatory disorders (9). It contributes to the development of type 2diabetes through its effects on insulin resistance and fatty acidmetabolism (10, 11). |
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References
Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. | |
Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. | |
White, A.M. et al. (1997) Am. J. Physiol. 273:L524. | |
Tang, P. et al. (1996) Biochemistry 35:8216. | |
Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. | |
Black, R.A. et al. (1997) Nature 385:729. | |
Moss, M.L. et al. (1997) Nature 385:733. | |
Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. | |
Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. | |
Romanatto, T. et al. (2007) Peptides 28:1050. | |
Hector, J. et al. (2007) Horm. Metab. Res. 39:250. | |
Long Name: | Tumor Necrosis Factor alpha |
Entrez Gene IDs: | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
Alternate Names: | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
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Related Research Areas
追加しました。
Related Product & Information
Background | TNF-alpha |
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background_content | Background: TNF-alpha Tumornecrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, isthe prototypic ligand of the TNF superfamily. It is a pleiotropicmolecule that plays a central role in inflammation, apoptosis, andimmune system development. TNF-alpha is produced by a wide variety of immuneand epithelial cell types (1, 2). Guinea pig TNF-alpha consists of a 35amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a155 aa extracellular domain (ECD) (3). Within the ECD, guinea pig TNF-alphashares 80% - 83% aa sequence identity with human, mouse, and ratTNF-alpha. The 26 kDa type 2 transmembrane protein is assembledintracellularly to form a noncovalently linked homotrimer (4). Ligationof this complex induces reverse signaling that promotes lymphocyteco-stimulation but diminishes monocyte responsiveness (5). Cleavage ofmembrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimericform of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and thehematopoietic cell-restricted TNF RII, both of which are also expressedas homotrimers (1, 8). TNF-alpha regulates lymphoid tissue developmentthrough control of apoptosis (2). It also promotes inflammatoryresponses by inducing the activation of vascular endothelial cells andmacrophages (2). TNF-alpha is a key cytokine in the development of severalinflammatory disorders (9). It contributes to the development of type 2diabetes through its effects on insulin resistance and fatty acidmetabolism (10, 11). |
追加しました。
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