抗Rat TNF-alpha抗体(Anti-Rat TNF-alpha antibody)
掲載日情報：2021/01/28 現在Webページ番号：187385
Rat TNF-alphaに対する抗体(Anti-Rat TNF-alpha )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
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Anti-Rat TNF-alpha MAb (Clone 45418R) |
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Anti-Rat TNF-alpha MAb (Clone 45418R) |
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[在庫・価格 ：2025年04月26日 20時35分現在]
Anti-Rat TNF-alpha MAb (Clone 45418R)
文献数： 0
- 商品コード：MAB510R-01M
- メーカー：RSD
- 包装：1mg
- 価格：ご照会ください
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：APC1 protein クローン：45418R Genbank No： 7124 Protein Accession No： P16599 |
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Rat | 適用 | ELISA,Neutralising |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Rat TNF-alpha MAb (Clone 45418R)
文献数： 0
- 商品コード：MAB510R-100
- メーカー：RSD
- 包装：100μg
- 価格：¥103,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat TNF-α サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF510，スタンダードとして#510-RT-010を用いる。 別名：APC1 protein クローン：45418R Genbank No： 7124 Protein Accession No： P16599 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Rat | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Recombinant | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Rat TNF-alpha MAb (Clone 45418R)
文献数： 0
- 商品コード：MAB510R-500
- メーカー：RSD
- 包装：500μg
- 価格：¥245,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：APC1 protein クローン：45418R Genbank No： 7124 Protein Accession No： P16599 |
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|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Rat | 適用 | ELISA,Neutralising |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
Anti-Rat TNF-alpha MAb (Clone 45418R)
文献数： 0
- 商品コード：MAB510R-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。 別名：APC1 protein クローン：45418R Genbank No： 7124 Protein Accession No： P16599 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Rat | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Recombinant | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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追加しました。
Product Details
| Species Reactivity | Rat |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant rat TNF-alpha Accession # P16599 |
| Source | Recombinant Monoclonal Mouse IgG1 Clone # 45418R |
| Purification | Protein A or G purified from cell culture supernatant |
| Specificity | Detects rat TNF-alpha in direct ELISAs. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF‑ alpha and less than 0.2% cross-reactivity with rhTNF‑ alpha, rpTNF‑ alpha, and rhTNF‑ beta. |
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Rat TNF-alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat TNF‑ alpha Antibody (Catalog #MAB510R ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat TNF‑ alpha Biotinylated Antibody (Catalog #BAF510 ) |
| ELISA Standard | Recombinant Rat TNF-alpha Protein (Catalog #510-RT ) | |
| Neutralization | Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 0.025 ng/mL Recombinant Rat TNF‑ alpha and 0.5 µg/mL Actinomycin D. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization TNF-alpha Antibody (45418R) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/TNFalpha_MAB510R_Block_Neutralize_19945.jpg) click image to view larger | Cytotoxicity Induced by TNF-alpha and Neutralization by Rat TNF-alpha Antibody.Recombinant Rat TNF-alpha (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Rat TNF-alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-alpha Recombinant Monoclonal Antibody (Catalog # MAB510R). The ND50 is typically 10-40 µg/mL in the presence of the metabolic inhibitor Actinomycin D (0.5 µg/mL, Catalog # 1229). |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after opening. | ||
| 6 months, -20 to -70 °C under sterile conditions after opening. | ||
| Background: TNF-alpha | Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69-76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11). |
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References
| Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. | |
| Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. | |
| Estler, H.C. et al. (1992) Biol. Chem. Hoppe-Seyler 373:271. | |
| Tang, P. et al. (1996) Biochemistry 35:8216. | |
| Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. | |
| Black, R.A. et al. (1997) Nature 385:729. | |
| Moss, M.L. et al. (1997) Nature 385:733. | |
| Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. | |
| Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. | |
| Romanatto, T. et al. (2007) Peptides 28:1050. | |
| Hector, J. et al. (2007) Horm. Metab. Res. 39:250. | |
| Long Name: | Tumor Necrosis Factor alpha |
| Entrez Gene IDs: | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
| Alternate Names: | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
追加しました。
Related Research Areas
| Neutralization | |
|---|---|
 | Cytotoxicity Induced by TNF-alpha and Neutralization by Rat TNF-alpha Antibody. Recombinant Rat TNF-alpha (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Rat TNF-alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-alpha Recombinant Monoclonal Antibody (Catalog # MAB510R). The ND50 is typically 10-40 µg/mL in the presence of the metabolic inhibitor Actinomycin D (0.5 µg/mL, Catalog # 1229). |
追加しました。
Related Product & Information
| Long Name | Tumor Necrosis Factor alpha |
|---|---|
| Entrez Gene IDs | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
| Background | TNF-alpha |
| background_content | Background: TNF-alpha Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69-76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11). |
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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