抗Mouse IL-4抗体(Anti-Mouse IL-4 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-4His23-Ser140Accession # P07750
Source	Monoclonal Rat IgG1 Clone # 30340
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-4 in ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL‑4 (Catalog # 404-ML) under non-reducing conditions only
Mouse IL-4 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑4 Antibody (Catalog #MAB404 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑4 Biotinylated Antibody (Catalog #BAF404 )
ELISA Standard		Recombinant Mouse IL-4 Protein (Catalog #404-ML )
Neutralization	Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.1-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody.Recombinant Mouse IL‑4 (Catalog # 404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑4 Monoclonal Antibody (Catalog # MAB404). The ND50 is typically 0.1-0.6 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4	Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11‑14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

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References

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.
Noma, Y. et al. (1986) Nature 319:640.
Redfield, C. et al. (1991) Biochemistry 30:11029.
Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
Corthay, A. (2006) Scand. J. Immunol. 64:93.
Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name:	Interleukin 4
Entrez Gene IDs:	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names:	B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

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Related Research Areas

Show

Common gamma Chain Receptor Family
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Cytokines and Receptors in Tfh Cell Differentiation and Function
Dendritic Cell Development
gamma delta T Cells
Group 2 ILCs
Inflammatory Mediators
Multiple Sclerosis and Myelin Related Molecules
Natural Killer T (NKT) Cells
Regulatory T Cells (Tregs)
T Cell Cytokine Signaling
Th2 Cells
Th9 Cells
Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis Authors: Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju PLoS ONE, 2018;13(6):e0198740. Species: Mouse Sample Type: Whole Cells Application: Functional Assay
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Excellent		Functional Assay	Human	Anonymous	05/10/2017

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Summary

Application	Functional Assay
Sample Tested	Peripheral blood neutrophils
Species	Human

Neutralization

Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody. Recombinant Mouse IL‑4 (Catalog # 404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-MouseIL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑4 Monoclonal Antibody (Catalog # MAB404). The ND50 is typically 0.1-0.6 µg/mL.

目次に戻る

Related Product & Information

Long Name	Interleukin 4
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Background	IL-4
background_content	Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11‑14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

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Citations

1. Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis
   Authors: Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju
   PLoS ONE, 2018;13(6):e0198740.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


2. Armc5 deletion causes developmental defects and compromises T-cell immune responses
   Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu
   Nat Commun, 2017;8(0):13834.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. ROR inverse agonist suppresses insulitis and prevents hyperglycemia in a mouse model of type 1 diabetes.
   Authors: Solt L, Banerjee S, Campbell S, Kamenecka T, Burris T
   Endocrinology, 2015;156(3):869-81.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
   Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
   J Biol Chem, 2015;290(37):22338-51.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. Basophils control T-cell responses and limit disease activity in experimental murine colitis.
   Authors: Gomez M, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, Goebel N, Schmidbauer K, Dunger N, Bruhl H, Renner K, Syed S, Mack M
   Mucosal Immunol, 2014;7(1):188-99.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.
   Authors: Reba S, Li Q, Onwuzulike S, Ding X, Karim A, Hernandez Y, Fulton S, Harding C, Lancioni C, Nagy N, Rodriguez M, Wearsch P, Rojas R
   Eur J Immunol, 2014;44(5):1410-21.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


7. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
   Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
   Eur. J. Immunol., 2012;42(5):1250-60.
   Species: Mouse
   Sample Type: Pleural Lavage Fluid
   Application: ELISA Development


8. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


9. The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
   Authors: Jin W, Qi S, Luo H
   BMC Immunol., 2011;12(0):68.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


10. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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