抗Human CD3 epsilon抗体(Anti-Human CD3 epsilon antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Human thymocytes (1)
Source	Monoclonal Mouse IgG1 Clone # UCHT1
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunoprecipitation	McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York
CyTOF-reported	Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
T Cell Stimulation	This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).

Flow Cytometry
	Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
	CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Entrez Gene IDs	916 (Human); 12501 (Mouse)
Background	CD3 epsilon
background_content	Background: CD3 epsilon CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

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Citations

1. ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function
   Authors: C Tao, H Shao, W Zhang, H Bo, F Wu, H Shen, S Huang
   Mol Med Rep, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


2. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
   Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
   Sci Rep, 2017;7(1):6607.
   Species: Human
   Sample Type: Whole Cells
   Application: Fuctional Assay


3. PLC?1 regulates SDF-1?-induced lymphocyte adhesion and migration to sites of inflammation
   Authors: M Strazza, I Azoulay-Al, M Peled, AV Smrcka, EY Skolnik, S Srivastava, A Mor
   Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue
   Authors: C Caër, C Rouault, T Le Roy, C Poitou, J Aron-Wisne, A Torcivia, JC Bichet, K Clément, M Guerre-Mil, S André
   Sci Rep, 2017;7(1):3000.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
   Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
   Arthritis Res. Ther., 2017;19(1):97.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


6. Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2
   Authors: Xun Qu
   Cell. Mol. Immunol., 2016;13(5):615-27.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts
   Authors: Elena Neumann
   Matrix Biol., 2016;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines.
   Authors: Huang W, Lu C, Wu Y, Ouyang S, Chen Y
   J Exp Clin Cancer Res, 2015;34(0):54.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


9. Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation.
   Authors: Moore C, Cui Q, Warsi N, Durafourt B, Zorko N, Owen D, Antel J, Bar-Or A
   J Immunol, 2015;194(2):761-72.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


10. PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation.
    Authors: Patsoukis N, Bardhan K, Chatterjee P, Sari D, Liu B, Bell L, Karoly E, Freeman G, Petkova V, Seth P, Li L, Boussiotis V
    Nat Commun, 2015;6(0):6692.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay

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