抗Human IL-10抗体(Anti-Human IL-10 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL‑10Ser19-Asn178Accession # P22301
Source	Monoclonal Mouse IgG2B Clone # 23738
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-10 in Western blots. In Western blots, this antibody shows approximately 5% cross-reactivity with recombinant mouseIL-10.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	10 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. At 2 µg/mL, this antibody will neutralize >60% of the bioactivity due to 5 ng/mL Recombinant Human IL‑10.

Western Blot
	Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization
	Cell Proliferation Induced by IL‑10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217). At 5 µg/mL, this antibody will neutralize 60% Recombinant Human IL-10 induced bioactivity.
Simple Western
	Detection of Human IL‑10 by Simple WesternTM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 at approximately 23 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Background	IL-10
background_content	Background: IL-10 Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

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Citations

1. The therapeutic HIV Env C5/gp41 vaccine candidate Vacc-C5 induces specific T cell regulation in a phase I/II clinical study
   Authors: K Brekke, M Sommerfelt, M Ökvist, AM Dyrhol-Rii, D Kvale
   BMC Infect. Dis, 2017;17(1):228.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
   Authors: BD Hock, SA MacPherson, JL McKenzie
   PLoS ONE, 2017;12(3):e0172858.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages
   Sci Rep, 2016;6(0):31959.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


4. Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
   Authors: Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
   PLoS ONE, 2014;9(2):e88327.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


5. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
   Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
   Nat Commun, 2014;5(0):3199.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
   Authors: Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M
   Eur J Immunol, 2014;44(6):1615-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
   Authors: Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C
   Eur. J. Immunol., 2008;38(12):3365-75.
   Species: Human
   Sample Type: Recombinant Protein
   Application: WB


8. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


9. Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
   Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
   J. Virol., 2007;81(11):5882-92.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Induction of host chemotactic response by Encephalitozoon spp.
    Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
    Infect. Immun., 2007;75(4):1619-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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