抗ATF4, C-term抗体 | Anti-ATF4, C-term antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

WB analysis of K562 lysate using GTX89973 ATF4 antibody, C-term. Dilution : 0.3μg/mlLoading : 35μg protein in RIPA buffer

目次に戻る

製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Rat

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.3-1μg/ml

Not tested in other applications.

Calculated MW	39 kDa. ( Note )
Positive Control	The peptide used to generate this antibody is available for purchase (GTX89973-PEP).
Specificity/Sensitivity	This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).
Predict Reactivity	Pig(>80% identity)

目次に戻る

PROPERTIES

Form	Liquid
Buffer	Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide with sequence C-EEVRKARGKKRVP, from the C Terminus of the protein sequence according to NP_001666.2; NP_877962.1.
Purification	Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	Activating Transcription Factor 4 , Creb-2 , Creb2 , Taxreb67 , Txreb , Atf4
Cellular Localization	Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
Background	This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]
Database	・ Gene ID: 468 ・ UniProt: P18848

目次に戻る

REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wu PS et al. PLoS One 2015; 10 (6) : e0130599 Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
Lin MH et al. Toxicology 2014; 315 : 24-37 Lipid peroxidation end product 4-hydroxy-trans-2-nonenal triggers unfolded protein response and heme oxygenase-1 expression in PC12 cells: Roles of ROS and MAPK pathways.	Application : WB Reactivity : Rat
Weng CY et al. Arch Toxicol 2014; 88 (2) : 213-26 * induces unfolded protein response in vascular endothelial cells.	Application : WB Reactivity : Mouse

目次に戻る