抗Hec1 [9G3.23]抗体 | Anti-Hec1 [9G3.23] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
	Confocal immunofluorescence staining (Olympus FV10i) of HEC1, a kinetochore outer layer marker. U2OS cells were fixed by 4% PFA and costained with Hec1 9G3.23 AB (Red; cat# GTX70268; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
	HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).
	Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
	Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.Blue: Hoechst 33342 staining.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	9G3.23
Isotype	IgG2a
Application	WB, ICC/IF, IHC-Fr, FACS, IP, PLA
Reactivity	Human, Hamster, Kangaroo rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	Assay dependent
FACS	Assay dependent
IP	Assay dependent
PLA	Assay dependent

Not tested in other applications.

Calculated MW	80 kDa. ( Note )
Positive Control	293T , A431 , HepG2 , HeLa, U87-MG NE nuclear extract

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	3.44 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human HEC1 protein consisting of amino acids 56-642.
Purification	Affinity purified by Protein A.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Ndc80, Kinetochore Complex Component , Hec , Hec1 , Hshec1 , Kntc2 , Tid3 , Hsndc80 , Ndc80
Cellular Localization	Nuclear
Background	Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation.
Database	・ Gene ID: 10403 ・ UniProt: O14777

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Kim S et al. Nat Commun 2020; 11 (1) : 612 PRMT6-mediated H3R2me2a guides Aurora B to chromosome arms for proper chromosome segregation.	Application : ICC/IF Reactivity : Human
Gwon D et al. Mol Cells 2019; 42 (12) : 840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.	Application : WB
Asai Y et al. J Cell Biol 2019; 218 (10) : 3223-3236 Aurora B kinase activity is regulated by SET/TAF1 on Sgo2 at the inner centromere.	Application : WB Reactivity : Human
Kim S et al. Toxicol In Vitro 2019; 59 : 115-125 Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.	Application : ICC/IF Reactivity : Human
Hayward D et al. J Cell Biol 2019; (Epub) CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization.	Application : ICC/IF Reactivity : Human

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