Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit | rTIM-1/KIM-1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111396
R&D Systems製のRat TIM-1/KIM-1/HAVCRを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月26日 00時00分現在]
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TIM-1, Rat, ELISA Kit, Quantikine (96well) <KIM-1, ELISA Kit> <HAVCR, ELISA Kit> |
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[在庫・価格 ：2024年04月26日 00時00分現在]
TIM-1, Rat, ELISA Kit, Quantikine (96well) <KIM-1, ELISA Kit> <HAVCR, ELISA Kit>
文献数： 5
- 商品コード：RKM100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：7.81 - 500 pg/mL，感度：2.74 pg/mL，測定試料：Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL)，種交差性：Rat，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：TIM-1/KIM-1/HAVCR 別名：CD365 Genbank No： 26762 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2022年11月15日号 p.6
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| 製品記事 |
Rat TIM-1/KIM-1/HAVCR Quantikine® ELISA Kit Quantikine TIM-1 ELISA Kit Immune Checkpoint Protein ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL)
- Sensitivity
- 2.74 pg/mL
- Assay Range
- 7.81 - 500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates, Urine)
- Specificity
- Natural and recombinant rat TIM-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Rat TIM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat TIM-1 in cell culture supernates, tissue homogenates, serum, plasma, and urine. It contains NS0-expressed recombinant rat TIM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat TIM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat TIM-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 21.8 | 54.4 | 161 | 26.2 | 60 | 169 |
| Standard Deviation | 0.8 | 2.45 | 5.22 | 2.32 | 3.79 | 7.51 |
| CV% | 3.7 | 4.5 | 3.2 | 8.9 | 6.3 | 4.4 |
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Recovery
The recovery of rat TIM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 102 | 82-112 |
| Heparin Plasma (n=4) | 102 | 95-114 |
| Cell Culture Supernates (n=5) | 96 | 93-99 |
| EDTA Plasma (n=4) | 99 | 84-107 |
| Urine (n=4) | 103 | 87-117 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with rat TIM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- TIM-1/KIM-1/HAVCR
- Aliases
- HAVCR1; KIM-1
- Entrez Gene IDs
- 26762 (Human); 171283 (Mouse); 286934 (Rat)
- Background
- TIM-1/KIM-1/HAVCR
- TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2 cell mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain. The ECD contains one V-type Ig like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation.The TIM-1 gene is highly polymorphic and undergoes alternate splicing. For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A. There are two cytoplasmic alternate splice forms of TIM-1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in TIM-1a. TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule. The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin. TIM1 ligation induces T cell proliferation and promotes cytokine production.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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