Rat IL-6 Quantikine ELISA Kit | rIL-6 Qkit
掲載日情報：2017/09/01 現在Webページ番号：111388
R&D Systems製のRat IL-6を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月04日 20時55分現在]
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IL-6, Rat, ELISA Kit, Quantikine, 2nd Generation (96 well) |
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[在庫・価格 ：2024年05月04日 20時55分現在]
IL-6, Rat, ELISA Kit, Quantikine, 2nd Generation (96 well)
文献数： 147
- 商品コード：R6000B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：62.5-4,000 pg/mL ，感度：36 pg/mL，測定試料：培養上清, 血清, 血漿(EDTA)，種交差性：Rat，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-6 別名：B cell stimulatory factor-2 Genbank No： 3569 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | 医薬用外毒物 | ||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2019年11月15日号 p.13
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| 製品記事 |
Quantikine IL-6 ELISA Kit Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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- ELISA kitトラブルシューティングガイド／FAQ
- Product Details
- Product Summary
- Precision
- Recovery
- Linearity
- Additional Infomation
- Citations
ELISA kitトラブルシューティングガイド／FAQ
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL)
- Sensitivity
- 36 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant rat IL-6
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Rat IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-6 in cell culture supernates, rat serum, and plasma. It contains E. coli-expressed recombinant rat IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat IL-6 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-6.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 27 | 28 |
| Mean | 120 | 256 | 1,028 | 134 | 268 | 987 |
| Standard Deviation | 10.6 | 22.4 | 46.2 | 13.4 | 21.7 | 69.2 |
| CV% | 8.8 | 8.8 | 4.5 | 10 | 8.1 | 7 |
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Recovery
The recovery of rat IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=5) | 103 | 85-118 |
| Serum (n=5) | 97 | 91-105 |
| EDTA Plasma (n=5) | 96 | 81-111 |
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Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-6
- Aliases
- BSF-2; IFN-beta 2; MGI-2A
- Entrez Gene IDs
- 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
- Background
- IL-6
- Interleukin 6 (IL-6), also known as interferon-beta 2, 26 kDa protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa - 28 kDa phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
Cytokine 2013 61:614-21
Species: Rat
Sample Type: BALF
Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture.
Casteleyn C, Francque S, Kwanten W, Laleman W, Van Dyck C, Van Steenkiste C, Verbeke L
Lab Invest 2012 92:1428-39
Species: Rat
Sample Type: Serum
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
J Neuroinflammation 2012 9:181
Vipera aspis venom reduces lethality and down-regulates tumor necrosis factor-alpha in a rat model of LPS-induced sepsis.
Adi N, Dahan E, Frolkis I, Klein Y, Locker C, Shapira I, Sorkine P, Uretzsky G
Cytokine 2010 49:319-24
Species: Rat
Sample Type: Serum
Mechanism of hepatoprotection in proestrus female rats following trauma-hemorrhage: heme oxygenase-1-derived normalization of hepatic inflammatory responses.
Bland KI, Chaudry IH, Chen J, Choudhry MA, Hu S, Rue LW, Yang S
J. Leukoc. Biol. 2009 85:1015-26
Species: Rat
Sample Type: Serum
Hypercapnic acidosis attenuates shock and lung injury in early and prolonged systemic sepsis.
Chonghaile MN, Contreras M, Costello J, Hassett P, Higgins B, Laffey JG, O'Toole D
Crit. Care Med. 2009 37:2412-20
Species: Rat
Sample Type: BALF
In vivo Angiotensin II AT1 receptor blockade selectively inhibits LPS-induced innate immune response and ACTH release in rat pituitary gland.
Benicky J, Pavel J, Saavedra JM, Sanchez-Lemus E
Brain Behav. Immun. 2009 23:945-57
Species: Rat
Sample Type: Plasma
Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha.
Kim HT, Kokorina N, Ma Y, Messina JL, Wang P, Xu J, Zhai L, Zhao L
Endocrinology 2008 149:2369-82
Species: Rat
Sample Type: Serum
Induction of type 2 iodothyronine deiodinase in the mediobasal hypothalamus by bacterial lipopolysaccharide: role of corticosterone.
Fekete C, Lechan RM, Sanchez E, Singru PS
Endocrinology 2008 149:2484-93
Species: Rat
Sample Type: Serum
Antithrombin inhibits bronchoalveolar activation of coagulation and limits lung injury during Streptococcus pneumoniae pneumonia in rats.
Bresser P, Choi G, Florquin S, Hofstra JJ, Levi M, Rijneveld AW, Roelofs JJ, Schultz MJ, van der Poll T, van der Zee JS
Crit. Care Med. 2008 36:204-10
Species: Rat
Sample Type: BALF
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