Porcine IL-6 Quantikine ELISA Kit | pIL-6 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111373
R&D Systems製のPorcine IL-6を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月30日 00時00分現在]
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IL-6, Porcine, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月30日 00時00分現在]
IL-6, Porcine, ELISA Kit, Quantikine (96well)
文献数： 52
- 商品コード：P6000B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 使用文献あり。測定範囲：18.8-1,200 pg/mL，感度：4.3 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Porcine，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-6 別名：B cell stimulatory factor-2 Genbank No： 3569 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | IL-6 ELISA Kit（R&D Systems 社） Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 4.3 pg/mL
- Assay Range
- 18.8 - 1,200 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant porcine IL-6
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Porcine IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IL-6 and antibodies raised against the recombinant factor. The immunoassay has been shown to accurately quantitate recombinant porcine IL-6. Results obtained using natural porcine IL-6 show dose response curves that are parallel to the standard curves obtained using recombinant Quantikine Porcine IL-6 kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-6.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 54.5 | 123 | 674 | 52.9 | 127 | 665 |
| Standard Deviation | 2.8 | 5.4 | 17.6 | 3.9 | 7.5 | 30.1 |
| CV% | 5.1 | 4.4 | 2.6 | 7.4 | 5.9 | 4.5 |
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Recovery
The recovery of porcine IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 102 | 95-109 |
| Serum (n=4) | 96 | 87-110 |
| EDTA Plasma (n=4) | 97 | 89-104 |
| Heparin Plasma (n=4) | 97 | 85-103 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of porcine IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-6
- Aliases
- BSF-2; IFN-beta 2; MGI-2A
- Entrez Gene IDs
- 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
- Background
- IL-6
- Interleukin 6 (IL-6), also known as interferon-beta 2, 26 kDa protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa - 28 kDa phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs.
Agusti C, de la Bellacasa JP, Esperatti M, Filella X, Guerrero L, Li Bassi G, Liapikou M, Luque N, Marco F, Martinez-Olondris P, Quera MA, Rigol M, Soy D, Torres A
Crit. Care Med. 2012 40:162-8
Species: Porcine
Sample Type: Serum
CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
Barratt-Due A, Castellheim A, Fosse E, Fure H, Hellerud BC, Johansen HT, Lindstad JK, Mollnes TE, Nielsen EW, Pharo A, Thorgersen EB, Tonnessen TI
FASEB J. 2010 24:712-22
Species: Porcine
Sample Type: Plasma
Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
Kurundkar AR, McILwain RB, Timpa JG
Lab. Invest. 2010 90:128-39
Species: Porcine
Sample Type: Plasma
Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
Brandtzaeg P, Castellheim A, Hellerud BC, Lindstad JK, Mollnes TE, Nielsen EW, Pharo A, Thorgersen EB, Tonnessen TI
Crit. Care Med. 2010 38:1467-74
Species: Porcine
Sample Type: Plasma
Mutations in classical swine fever virus NS4B affect virulence in swine.
Fernandez-Sainz I, Gladue DP, Holinka LG
J. Virol. 2010 84:1536-49
Species: Porcine
Sample Type: Cell Culture Supernates
Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
Jeong HJ, Lee SW, Song YJ
Clin. Vaccine Immunol. 2010 17:503-12
Species: Porcine
Sample Type: Cell Culture Supernates
Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
Axelsen AK, Gaustad P, Haverson K, Kotwal GJ, Mollnes TE, Pharo A, Sfyroera G, Thorgersen EB
Infect. Immun. 2009 77:725-32
Species: Porcine
Sample Type: Whole Blood
Bbeta15-42 (FX06) reduces pulmonary, myocardial, liver, and small intestine damage in a pig model of hemorrhagic shock and reperfusion.
Iber T, Koch A, Noldge-Schomburg GE, Petzelbauer P, Roesner JP, Scheeren TW, Tran N, Vagts DA, Vollmar B, Zacharowski K
Crit. Care Med. 2009 37:598-605
Species: Porcine
Sample Type: Plasma
Effect of linezolid compared with glycopeptides in methicillin-resistant Staphylococcus aureus severe pneumonia in piglets.
Absi R, Baleztena M, Biancolini C, Bruno DA, Famiglietti A, Garcia Bottino M, Garcia-Morato J, Luna CM, Mann KC, Marchetti D, Risso Patron J, Sagardia J
Chest 2009 135:1564-71
Species: Porcine
Sample Type: Serum
Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
Filler SG, Lewis RJ, Niemann JT, Phan QT, Rosborough JP, Shah AP, Youngquist S
J. Interferon Cytokine Res. 2009 0:
Species: Porcine
Sample Type: Plasma
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