Porcine IL-10 Quantikine ELISA Kit | pIL-10 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111371
R&D Systems製のPorcine IL-10を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月13日 00時00分現在]
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IL-10, Porcine, ELISA Kit, Quantikine P (96 well) |
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[在庫・価格 ：2025年05月13日 00時00分現在]
IL-10, Porcine, ELISA Kit, Quantikine P (96 well)
文献数： 15
- 商品コード：P1000
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：31.3-2,000 pg/mL，感度：5.5 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Porcine，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-10 別名：CSIF Genbank No： 3586 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL)
- Sensitivity
- 5.5 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant porcine IL-10
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Porcine IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-10 levels in cell culture supernates, porcine serum, and EDTA plasma. It contains E. coli-expressed recombinant porcine IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring porcine IL-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural porcine IL-10.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 88 | 264 | 791 | 89 | 264 | 802 |
| Standard Deviation | 3.7 | 6.8 | 27.5 | 6.4 | 11.8 | 54.7 |
| CV% | 4.2 | 2.6 | 3.5 | 7.2 | 4.5 | 6.8 |
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Recovery
The recovery of porcine IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 94 | 84-102 |
| Cell Culture Supernates (n=4) | 106 | 101-109 |
| EDTA Plasma (n=4) | 92 | 80-100 |
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Linearity
To assess the linearity of the assay, four samples spiked with high concentrations of porcine IL-10 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-10
- Aliases
- CSIF
- Entrez Gene IDs
- 3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
- Background
- IL-10
- Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper (Th) 2 clones that inhibited cytokine production by Th1 clones . The human ortholog of mouse IL-10 was subsequently cloned by cross-hybridization. Human and mouse IL-10 are 81% identical at the nucleotide and amino acid level. IL-10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. A number of viruses, including Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV), also encode viral members of the IL-10 family. Human IL-10 has cross-species actvity and is active on mouse cells. Mouse IL-10 is species-specific and does not act on human cells.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota.
Jeffery IB, Riboulet-Bisson E, Sturme MH
PLoS ONE 2012 7:e31113
Species: Porcine
Sample Type: Serum
Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
Jeong HJ, Lee SW, Song YJ
Clin. Vaccine Immunol. 2010 17:503-12
Species: Porcine
Sample Type: Cell Culture Supernates
Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
Axelsen AK, Gaustad P, Haverson K, Kotwal GJ, Mollnes TE, Pharo A, Sfyroera G, Thorgersen EB
Infect. Immun. 2009 77:725-32
Species: Porcine
Sample Type: Whole Blood
Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
Coakley M, Fitzgerald GF, Hart O, Kiely B, Lawlor P, O'Mahony C, O'Mahony L, Quigley EM, Ross RP, Shanahan F, Stanton C, Wall R
Am. J. Clin. Nutr. 2009 89:1393-401
Species: Porcine
Sample Type: Cell Culture Supernates
Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
Haraldsen G, Lambris JD, Magotti P, Mollnes TE, Saethre M, Schneider MK, Seebach JD
J. Immunol. 2008 180:6346-53
Species: Porcine
Sample Type: Cell Culture Supernates
Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
Essen-Gustavsson B, Fossum C, Jensen-Waern M, Kruse R
Acta Vet. Scand. 2008 50:32
Species: Porcine
Sample Type: Serum
Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation.
Absi R, Agusti C, Baquero S, Famiglietti A, Gando S, Luna CM, Morato JG, Patron JR, Sibila O, Torres A, Vay CA, Von Stecher F
Chest 2007 132:523-31
Species: Porcine
Sample Type: Serum
Butyrate differentially regulates cytokines and proliferation in porcine peripheral blood mononuclear cells.
Kerr BJ, Weber TE
Vet. Immunol. Immunopathol. 2006 113:139-47
Species: Porcine
Sample Type: Cell Culture Supernates
Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide.
Changhua L, Defa L, Jianjun X, Jindong Y, Lidan Z, Shiyan Q
J. Nutr. 2005 135:239-44
Species: Porcine
Sample Type: Serum
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