Mouse ST2/IL-1 R4 Quantikine ELISA Kit | mST2 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111356
R&D Systems製のMouse ST2/IL-1 R4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2026年04月05日 00時00分現在]
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ST2/IL-33 R, Mouse, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2026年04月05日 00時00分現在]
ST2/IL-33 R, Mouse, ELISA Kit, Quantikine (96well)
文献数： 9
- 商品コード：MST200
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.156-10 ng/mL，感度：0.059 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：ST2, IL-33R 別名：DER4 Genbank No： 9173 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.059 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse ST2
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Mouse ST2/IL-1 R4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ST2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse ST2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ST2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.465 | 1.34 | 3.81 | 0.512 | 1.43 | 3.94 |
| Standard Deviation | 0.019 | 0.042 | 0.106 | 0.047 | 0.082 | 0.138 |
| CV% | 4.1 | 3.1 | 2.8 | 9.2 | 5.7 | 3.5 |
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Recovery
The recovery of mouse ST2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 103 | 89-113 |
| Heparin Plasma (n=4) | 94 | 86-103 |
| Cell Culture Supernates (n=4) | 98 | 81-111 |
| EDTA Plasma (n=4) | 104 | 92-118 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- ST2/IL-1 R4
- Aliases
- DER4; Fit-1; IL-1RL1; IL-1 R4; IL-33R; Ly84; ST2L; ST2V; T1
- Entrez Gene IDs
- 9173 (Human); 17082 (Mouse)
- Background
- ST2
- The Interleukin 1 receptor family comprises at least nine members. All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. A subtype of the IL-1 R family, SIGIRR (single Ig domain-containing IL-1 R-related molecule) differs from the other nine members by having only one Ig domain.ST2L, also known as IL-1 R4, is a transmembrane glycoprotein in the interleukin-1 receptor family. ST2L is expressed on mast cells and Th2 cells as well as on stressed cardiac myocytes and airway epithelia. ST2L associates with IL-1R AcP to form a receptor complex for IL-33. Alternative splicing generates ST2, a secreted isoform that functions as an inhibitor of IL-33 signaling. Within the ECD, mouse ST2L shares 68% aa sequence identity with human ST2L.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Vasoactive intestinal peptide downregulates proinflammatory TLRs while upregulating anti-inflammatory TLRs in the infected cornea.
Barrett RP, Hazlett LD, Jiang X, McClellan SA, Zhang Y
J. Immunol. 2012 189:269-78
Species: Mouse
Sample Type: Tissue Homogenates
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