Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit | m/rSOST Qkit
掲載日情報：2016/08/18 現在Webページ番号：111355
R&D Systems製のMouse/Rat SOST/Sclerostinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Mouse/Rat SOST Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Mouse/Rat SOST Quantikine ELISA Kit
文献数： 9
- 商品コード：MSST00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：4.17 pg/mL，測定範囲：15.6-1,000 pg/mL，種交差性：Mouse/Rat，測定試料：Serum, Plasma, Cell Culture Supernatant，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：SOST, Sclerostin 別名：sclerostin Genbank No： 50964 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Mouse/Rat SOST Quantikine(R) ELISA Kit Quantikine SOST ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 4.17 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL
- Specificity
- Natural and recombinant mouse and rat SOST
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat SOST immunoassay is a 4.5 hour solid-phase ELISA designed to measure SOST in mouse or rat cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse SOST and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SOST. Results obtained using natural mouse or rat SOST showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SOST.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 57.6 | 108 | 345 | 54.4 | 101 | 347 |
| Standard Deviation | 4.15 | 6 | 18.7 | 4.35 | 6.23 | 19.4 |
| CV% | 7.2 | 5.6 | 5.4 | 8 | 6.2 | 5.6 |
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Recovery
The recovery of SOST spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 101 | 92-112 |
| Mouse Serum (n=4) | 95 | 86-113 |
| Mouse EDTA Plasma (n=4) | 98 | 82-113 |
| Mouse Heparin Plasma (n=4) | 102 | 88-115 |
| Rat Serum (n=4) | 99 | 85-116 |
| Rat EDTA Plasma (n=2) | 107 | 101-118 |
| Rat Heparin Plasma (n=2) | 96 | 82-111 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SOST in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- SOST/Sclerostin
- Aliases
- undefined
- Entrez Gene IDs
- 50964 (Human); 74499 (Mouse)
- Background
- SOST/Sclerostin
- SOST, also known as sclerostin, is a member of the Cerberus/DAN family of BMP antagonists, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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