Mouse/Rat IGF-I Quantikine ELISA Kit | m/rIGF-I/IGF-1 Qkit
掲載日情報：2017/09/01 現在Webページ番号：111301
R&D Systems製のMouse/Rat IGF-Iを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月02日 20時55分現在]
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IGF-I/IGF-1, Mouse/Rat, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年05月02日 20時55分現在]
IGF-I/IGF-1, Mouse/Rat, ELISA Kit, Quantikine (96well)
文献数： 141
- 商品コード：MG100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：8.4 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates，種交差性：Mouse/Rat，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IGF-I, IGF-1 別名：IBP1 Genbank No： 3479 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | 医薬用外毒物 | ||||||
| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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- ELISA kitトラブルシューティングガイド／FAQ
- Product Details
- Product Summary
- Precision
- Recovery
- Linearity
- Additional Infomation
- Citations
ELISA kitトラブルシューティングガイド／FAQ
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 8.4 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates)
- Specificity
- Natural and recombinant mouse and rat IGF-I
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat IGF-I Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IGF-I in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse IGF-I and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse and rat IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat IGF-I.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 35 | 35 | 35 |
| Mean | 82 | 269 | 921 | 86 | 256 | 863 |
| Standard Deviation | 4.6 | 8.9 | 37.7 | 7.8 | 11.8 | 37.4 |
| CV% | 5.6 | 3.3 | 4.1 | 9.1 | 4.6 | 4.3 |
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Recovery
The recovery of mouse/rat IGF-I spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.
| Sample Type | Average % Recovery | Range % |
| Mouse Cell Culture Supernates (n=7) | 98 | 89-111 |
| Rat Cell Culture Supernates (n=6) | 98 | 82-108 |
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Linearity
To assess the linearity of the assay, samples containing mouse or rat IGF-I in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IGF-I
- Aliases
- IGF1; IGF-1; Somatomedin A; Somatomedin C
- Entrez Gene IDs
- 3479 (Human); 16000 (Mouse); 24482 (Rat)
- Background
- IGF Ligands
- Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conservedbetween the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.
Aida , Takuya, Aono , Fumihito, Aoyama , Naoki, Ezoe , Kenji, Kato , Keiich, Kato , Osamu, Kuroda , Tomoko, Murata , Nana, Nakama , Ken, Sano , Chiaki, Takehara , Yuji, Yabuuchi , Akiko, Yamadera , Rie
Lab Invest 2013 93:181-93
Species: Rat
Sample Type: Cell Culture Supernates
BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer's disease.
Blusztajn J, Burke R, Haydar T, Leeman S, Mellott T, Norman T, Slack B
Proc Natl Acad Sci U S A 2013 110:19567-72
Species: Mouse
Sample Type: Tissue Homogenates
Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway.
Cheng YP, Hu YY, Ma B, Wang YL, Wu D, Yang ZD, Ying HJ, Zhang Q, Zheng YY
Acta Pharmacol. Sin. 2012 33:479-89
Cytosolic Malic Enzyme 1 (ME1) Mediates High Fat Diet-Induced Adiposity, Endocrine Profile, and Gastrointestinal Tract Proliferation-Associated Biomarkers in Male Mice.
Al-Dwairi , Ahmed, Pabona , John Mar, Simmen , Frank A, Simmen , Rosalia
PLoS ONE 2012 7:e46716
Species: Mouse
Sample Type: Serum
Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function.
Isogai Y, Masuda S, Sato H
J. Biol. Chem. 2011 286:11632-48
Species: Mouse
Sample Type: Serum
Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion.
Mishra S, Moulik S, Nguyen KH, Nyomba BL, Yao XH
Endocrinology 2011 152:2184-96
Species: Mouse
Sample Type: Plasma
Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy.
Bahr O, Brucker DP, Harter PN, Hattingen E, Maurer GD, Mueller-Klieser W, Rieger J, Steinbach JP, Walenta S
BMC Cancer 2011 11:315
Species: Mouse
Sample Type: Serum
Peripheral SMN restoration is essential for long-term rescue of a severe spinal muscular atrophy mouse model.
Hua Y, Rigo F, Sahashi K
Nature 2011 478:123-6
Species: Mouse
Sample Type: Serum
Galectin-3 contributes to neonatal hypoxic-ischemic brain injury.
Doverhag C, Hagberg H, Hedtjarn M, Karlsson A, Mallard C, Poirier F, Savman K
Neurobiol. Dis. 2010 38:36-46
Species: Mouse
Sample Type: Tissue Homogenates
Rim2alpha determines docking and priming States in insulin granule exocytosis.
Minami K, Shibasaki T, Yasuda T
Cell Metab. 2010 12:117-29
Species: Mouse
Sample Type: Serum
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