Mouse CXCL4/PF4 Quantikine ELISA Kit | mCXCL4/PF4 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111290
R&D Systems製のMouse CXCL4/PF4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月27日 12時15分現在]
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Platelet Factor 4, Mouse, ELISA Kit, Quantikine (96 well) <PF4, ELISA Kit> <CXCL4, ELISA Kit> |
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[在庫・価格 ：2024年04月27日 12時15分現在]
Platelet Factor 4, Mouse, ELISA Kit, Quantikine (96 well) <PF4, ELISA Kit> <CXCL4, ELISA Kit>
文献数： 4
- 商品コード：MCX400
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.08 - 5 ng/mL，感度：0.01 ng/mL，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：CXCL4/PF4 別名：chemokine (C-X-C motif) ligand 4 Genbank No： 5196 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Mouse CXCL4/PF4 Quantikine(R) ELISA Kit CXCL4 / PF4測定ELISAキット |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL)
- Sensitivity
- 0.01 ng/mL
- Assay Range
- 0.078 - 5 ng/mL (Serum, Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant mouse PF4
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse PF4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PF4 in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse PF4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse PF4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PF4.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.23 | 0.41 | 1.51 | 0.25 | 0.45 | 1.46 |
| Standard Deviation | 0.013 | 0.029 | 0.046 | 0.019 | 0.035 | 0.11 |
| CV% | 5.7 | 7.1 | 3 | 7.6 | 7.8 | 7.5 |
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Recovery
The recovery of mouse PF4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 102 | 90-120 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse PF4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CXCL4/PF4
- Aliases
- PF4
- Entrez Gene IDs
- 5196 (Human); 56744 (Mouse); 360918 (Rat)
- Background
- CXCL4/PF4 (Platelet Factor 4)
- Platelet factor 4 (PF4) is a member of the CXC chemokine family. Human and mouse PF4 share 64% identity. PF4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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