Mouse CCL17/TARC Quantikine ELISA Kit | mCCL17/TARC Qkit
掲載日情報：2016/08/18 現在Webページ番号：111275
R&D Systems製のMouse CCL17/TARCを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月04日 20時55分現在]
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TARC, Mouse, ELISA Kit, Quantikine M (96well) <CCL17, ELISA Kit> |
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[在庫・価格 ：2024年05月04日 20時55分現在]
TARC, Mouse, ELISA Kit, Quantikine M (96well) <CCL17, ELISA Kit>
文献数： 49
- 商品コード：MCC170
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：7.80 - 500 pg/mL，感度：5 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：CCL17/TARC 別名：ABCD-2 Genbank No： 6361 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
TARC Quantikine/DuoSet ELISA Kit Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL)
- Sensitivity
- 5 pg/mL
- Assay Range
- 7.8 - 500 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse TARC
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse TARC Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TARC levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TARC and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse TARC showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TARC.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 27 | 89 | 278 | 29 | 98 | 310 |
| Standard Deviation | 1.3 | 2.3 | 7.8 | 2.2 | 6.9 | 17.3 |
| CV% | 4.8 | 2.6 | 2.8 | 7.6 | 7 | 5.6 |
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Recovery
The recovery of mouse TARC spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 94 | 82-103 |
| Cell Culture Supernates (n=6) | 94 | 85-113 |
| EDTA Plasma (n=6) | 94 | 83-109 |
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Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse TARC in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- CCL17/TARC
- Aliases
- TARC
- Entrez Gene IDs
- 6361 (Human); 20295 (Mouse)
- Background
- CCL17/TARC (Thymus and Activation Regulated Chemokine)
- Thymus and activation-regulated chemokine (TARC) is a novel CC chemokine identified using a signal sequence trap method. Among CC chemokine family members, TARC has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. TARC is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
Aritake K, Hirai H, Nagata K, Nakamura M, Narumiya S, Otani S, Satoh T, Urade Y, Yamamoto Y, Yokozeki H
Am. J. Pathol. 2012 179:302-14
Species: Mouse
Sample Type: Tissue Homogenates
Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
J. Immunol. 2012 189:2545-52
IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
Christmann BS, Lowell CA, Metz AE, Nelson MP, Steele C, Trevor JL, Werner JL
J. Immunol. 2011 186:2372-81
Species: Mouse
Sample Type: Tissue Homogenates
Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
Li Y, Louten J, Rankin AL
Int. Immunol. 2011 23:307-15
Species: Mouse
Sample Type: BALF
Thymic stromal lymphopoietin is a key mediator of breast cancer progression.
Bodogai M, Olkhanud PB, Rochman Y
J. Immunol. 2011 186:5656-62
Species: Mouse
Sample Type: Plasma
IL-33-activated dendritic cells are critical for allergic airway inflammation.
Besnard AG, Erard F, Guillou N, Quesniaux V, Ryffel B, Togbe D
Eur. J. Immunol. 2011 41:1675-86
Species: Mouse
Sample Type: BALF
IL-22 Is Produced by Innate Lymphoid Cells and Limits Inflammation in Allergic Airway Disease.
Arnold-Schild D, Becher B, Buhl R, Dehzad N, Gyulveszi G, Kreymborg K, Michel E, Renauld JC, Reuter S, Schild H, Schneeweiss K, Taube C, Tertilt C
PLoS ONE 2011 6:e21799
Species: Mouse
Sample Type: BALF
Thymic stromal lymphopoietin is produced by dendritic cells.
Kashyap M, Rochman Y, Spolski R
J. Immunol. 2011 187:1207-11
Species: Mouse
Sample Type: BALF
Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
Ballantyne SJ, Barlow JL, Flynn RJ, McKenzie AN
Clin. Exp. Allergy 2011 41:1447-55
Species: Mouse
Sample Type: BALF
Murine atopic dermatitis responds to peroxisome proliferator-activated receptors alpha and beta/delta (but not gamma) and liver X receptor activators.
Crumrine D, Elias PM, Feingold KR, Hatano Y, Holleran WM, Man MQ, Mauro TM, Uchida Y
J. Allergy Clin. Immunol. 2010 125:160-9.e1-5
Species: Mouse
Sample Type: Serum
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