Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit | m/r/ca/pTGF-b2 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111269
R&D Systems製のMouse/Rat/Canine/Porcine TGF-beta 2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
TGF-β2, Canine/Mouse/Porcine/Rat, ELISA Kit, Quantikine
文献数： 5
- 商品コード：MB200
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：4.26 pg/mL，測定範囲：12.5-800 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Lysate，種交差性：Mouse/Rat/Porcine/Canine，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：TGF-beta 2 別名：BSC-1 cell growth inhibitor Genbank No： 7042 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine TGF-β2 ELISA Kit Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Cell Lysates (25 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
- Sensitivity
- 4.26 pg/mL
- Assay Range
- 12.5 - 800 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Recombinant and natural mouse, rat, and canine TGF-beta 2
- Interference
- No significant interference observed with available related molecules.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 29 | 102 | 323 | 29.9 | 115 | 337 |
| Standard Deviation | 2.43 | 4.97 | 21 | 2.8 | 8.2 | 30.7 |
| CV% | 8.4 | 4.9 | 6.5 | 9.4 | 7.2 | 9.1 |
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Recovery
The recovery of TGF-beta 2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 92 | 80-102 |
| Cell Lysates (n=2) | 94 | 86-98 |
| Serum (n=4) | 98 | 85-114 |
| EDTA Plasma (n=4) | 84 | 78-94 |
| Heparin Plasma (n=4) | 82 | 74-92 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of TGF-beta 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- TGF-beta 2
- Aliases
- TGFB2
- Entrez Gene IDs
- 7042 (Human); 21808 (Mouse); 397084 (Porcine)
- Background
- TGF-beta Family Ligands
- Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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