Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit | "m/r/p/caTGF-b1 Qkit, 2nd Gen"
掲載日情報：2017/09/01 現在Webページ番号：111268
R&D Systems製のMouse/Rat/Porcine/Canine TGF-beta 1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月09日 14時55分現在]
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TGF-β1, Canine/Mouse/Porcine/Rat, ELISA Kit, Quantikine, 2nd Generation (96well) |
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[在庫・価格 ：2025年05月09日 14時55分現在]
TGF-β1, Canine/Mouse/Porcine/Rat, ELISA Kit, Quantikine, 2nd Generation (96well)
文献数： 260
- 商品コード：MB100B
- メーカー：RSD
- 包装：1kit
- 本製品は製品内容等が変更されました
| 説明文 | Genbank No：
7040
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| 保存条件 | 法規備考 | ||
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| 製品記事 | Helper T Cells Marker Antibodies R&D Systems社製品#MB100Bの販売終了および改良版#DB100Cの販売 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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- ELISA kitトラブルシューティングガイド／FAQ
- Product Details
- Product Summary
- Precision
- Recovery
- Linearity
- Additional Infomation
- Citations
ELISA kitトラブルシューティングガイド／FAQ
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (40 µL), EDTA Plasma (40 µL), Heparin Plasma (40 µL)
- Sensitivity
- 15.4 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant TGF-beta 1. This kit also recognizes human TGF-beta 1.2.
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Mouse/Rat/Porcine/Canine TGF-beta 1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TGF-beta 1 in acid activated cell culture supernates, serum, and plasma. It contains recombinant TGF-beta 1 expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural TGF-beta 1 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural TGF-beta 1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 21 | 22 |
| Mean | 88 | 198 | 816 | 97 | 209 | 826 |
| Standard Deviation | 3 | 4.9 | 19.6 | 8.1 | 17.3 | 47 |
| CV% | 3.4 | 2.5 | 2.4 | 8.4 | 8.3 | 5.7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 28 | 37 | 30 |
| Mean | 78 | 180 | 695 | 79 | 185 | 730 |
| Standard Deviation | 2.3 | 3.4 | 17.3 | 7.2 | 17.2 | 46.8 |
| CV% | 2.9 | 1.9 | 2.5 | 9.1 | 9.3 | 6.4 |
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Recovery
The recovery of TGF-beta 1 spiked to levels throughout the range of the assay followed by activation was evaluated.
| Sample Type | Average % Recovery | Range % |
| Mouse Cell Culture Supernates (n=4) | 105 | 83-114 |
| Rat Cell Culture Supernates (n=3) | 112 | 93-125 |
| Porcine Cell Culture Supernates (n=2) | 109 | 102-118 |
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Linearity
To assess the linearity of the assay, the activated samples containing and/or spiked with high concentrations of TGF-beta 1 were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- TGF-beta 1
- Aliases
- TGFB1
- Entrez Gene IDs
- 7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine)
- Background
- TGF-beta Family Ligands
- Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Anti-inflammatory effects of FTY720 do not prevent neuronal cell loss in a rat model of optic neuritis.
Bahr M, Diem R, Hein K, Hillgruber C, Kretzschmar B, McRae BL, Rau CR, Sattler MB
Am. J. Pathol. 2012 178:1770-81
Species: Rat
Sample Type: Tissue Homogenates
Severe dermatitis with loss of epidermal Langerhans cells in human and mouse zinc deficiency.
Kawamura T, Nakamura Y, Ogawa Y
J. Clin. Invest. 2012 122:722-32
Species: Mouse
Sample Type: Cell Culture Supernates
Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.
Conlon H, Jun E, Muller AM, Sadiq SA
BMC Neurosci 2012 13:30
Species: Mouse
Sample Type: Serum
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Dervish S, Fulcher G, Harrison LC, Jackson CJ, Xue M
J. Biol. Chem. 2012 287:16356-64
Species: Mouse
Sample Type: Cell Culture Supernates
TLR-mediated distinct IFN-gamma/IL-10 pattern induces protective immunity against murine visceral leishmaniasis.
Eur. J. Immunol. 2012 42:2087-99
Therapeutic blockade of transforming growth factor beta fails to promote clearance of a persistent viral infection.
J. Virol. 2012 86:7060-71
Species: Mouse
Sample Type: Serum
Comparison of the effect of calcium gluconate and batroxobin on the release of transforming growth factor beta 1 in canine platelet concentrates.
Carmona J, Rezende C, Silva R
BMC Vet Res 2012 8:121
Species: Canine
Sample Type: Plasma
Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
Blood 2012 120:2600-9
Species: Mouse
Sample Type: tissue lysates
Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
Brown MA, Ehrenstein MR, Notley CA, Wright GP
J. Immunol. 2011 186:4967-72
Species: Mouse
Sample Type: Serum
Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
Diao J, Winter E, Zhao J
J. Immunol. 2011 186:5058-67
Species: Mouse
Sample Type: Cell Culture Supernates
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