Human CXCL8/IL-8 Quantikine HS ELISA Kit | hCXCL8/IL-8 QKit HS Kit
掲載日情報：2016/08/18 現在Webページ番号：111239
R&D Systems製のHuman CXCL8/IL-8 Quantikine HS ELISA Kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月04日 00時00分現在]
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IL-8, Human, ELISA Kit, Quantikine HS (96well) <CXCL8, ELISA Kit> |
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[在庫・価格 ：2024年05月04日 00時00分現在]
IL-8, Human, ELISA Kit, Quantikine HS (96well) <CXCL8, ELISA Kit>
文献数： 23
- 商品コード：HS800
- メーカー：RSD
- 包装：1kit
- 価格：¥124,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：0.4 pg/mL，測定範囲：1.00 - 64 pg/mL，種交差性：Human，測定試料：Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC41，測定時間：4.5 hours，測定因子：IL-8/CXCL8 別名：3-10C Genbank No： 3576 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine HS Human IL-8 ELISA Kit 新型コロナウイルス感染に起因するサイトカインストームのモニタリング用製品 |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 0.4 pg/mL
- Assay Range
- 1 - 64 pg/mL (Serum, Heparin Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-8
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine HS Human IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-8 levels in serum and plasma. It contains E. coli-expressed recombinant human IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-8 accurately. Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 5.5 | 18.7 | 37.1 | 5 | 19.4 | 39.2 |
| Standard Deviation | 0.3 | 0.7 | 2.7 | 0.4 | 1.6 | 3.7 |
| CV% | 5.5 | 3.7 | 7.3 | 8 | 8.2 | 9.4 |
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Recovery
The recovery of IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 99 | 85-110 |
| EDTA Plasma (n=4) | 95 | 86-102 |
| Heparin Plasma (n=4) | 99 | 91-107 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CXCL8/IL-8
- Aliases
- GCP1; IL8; LAI; MDNCF; NAP1; NCF; TCF; TSG1
- Entrez Gene IDs
- 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
- Background
- CXCL8/IL-8
- CXCL8 was originally discovered and purified as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocytederived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor-4, GRO, and IP10.CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other proinflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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