Human IL-12 p70 Quantikine HS ELISA Kit | hIL-12 p70 QKit HS Kit
掲載日情報：2016/08/18 現在Webページ番号：111235
R&D Systems製のHuman IL-12 p70 Quantikine HS ELISA Kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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IL-12, Human, ELISA Kit, Quantikine HS (96well) |
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[在庫・価格 ：2025年04月27日 00時00分現在]
IL-12, Human, ELISA Kit, Quantikine HS (96well)
文献数： 21
- 商品コード：HS120
- メーカー：RSD
- 包装：1kit
- 価格：¥124,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物,カルタヘナ該当品
| 説明文 | 測定範囲：0.625-40 pg/mL (C), 0.781-50 pg/mL (S, P)，感度：0.5 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：colorimetric，測定波長：490 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC41，測定時間：6.5 hours，測定因子：IL-12 p70 High Sensitivity 別名：CLMF p35 Genbank No： 3592 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | 医薬用外毒物,カルタヘナ該当品 | ||||||
| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | Human IL-12 ELISA Kit Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 6.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL)
- Sensitivity
- 0.5 pg/mL
- Assay Range
- 0.625 - 40 pg/mL (Cell Culture Supernates), 0.781 - 50 pg/mL (Serum, Heparin Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-12p70
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine HS Human IL-12 Immunoassay is a 6.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL-12 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 5.26 | 18.6 | 35.8 | 2.86 | 9.25 | 36.4 |
| Standard Deviation | 0.26 | 0.77 | 0.9 | 0.36 | 1.39 | 2.76 |
| CV% | 4.9 | 4.1 | 2.5 | 12.6 | 15 | 7.6 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 5.32 | 13 | 25 | 2.86 | 8.49 | 29.6 |
| Standard Deviation | 0.24 | 0.71 | 1.89 | 0.5 | 0.85 | 2.43 |
| CV% | 4.5 | 5.5 | 7.6 | 17.5 | 10 | 8.2 |
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Recovery
The recovery of IL-12 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 100 | 91-115 |
| Heparin Plasma (n=5) | 97 | 86-111 |
| EDTA Plasma (n=5) | 102 | 83-117 |
| Cell Culture Media (n=5) | 95 | 85-107 |
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Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-12 p70
- Aliases
- undefined
- Entrez Gene IDs
- 3592 (Human); 16159 (Mouse); 84405 (Rat)
- Background
- IL-12
- Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Human complete Stat-1 deficiency is associated with defective type I and II IFN responses in vitro but immunity to some low virulence viruses in vivo.
Arkwright PD, Casanova JL, Chapgier A, Feinberg J, Filipe-Santos O, Hawkins K, Jouanguy E, Wynn RF, Zhang S
J. Immunol. 2006 176:5078-83
Species: Human
Sample Type: Plasma
Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
Clark R, Dowgiert R, Eapen S, Hirahara K, Hurwitz D, Jones DA, Kupper TS, Mizutani H, Rich BE, Shibata M, Yamanaka K
Clin. Cancer Res. 2006 12:376-82
Species: Human
Sample Type: Plasma
Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients.
Bartosik-Psujek H, Stelmasiak Z
J Neural Transm 2005 112:797-803
Species: Human
Sample Type: Serum
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