Human VAP-1 Quantikine ELISA Kit | hVAP-1 Qkit

掲載日情報:2016/08/18 現在Webページ番号:111224

R&D Systems製のHuman VAP-1を測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2024年05月08日 00時01分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Human VAP-1 Quantikine ELISA Kit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 3
説明文
感度:0.077 ng/mL,測定範囲:0.78 - 50 ng/mL,種交差性:Human,測定試料:Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL),検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC182,測定時間:4.5 hours,測定因子:VAP-1/AOC3
別名:amine oxidase
Genbank No: 8639
法規制等 カルタヘナ該当品
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Quantikine Human VAP-1 ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

[在庫・価格 :2024年05月08日 00時01分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Human VAP-1 Quantikine ELISA Kit

文献数: 3

説明文 感度:0.077 ng/mL,測定範囲:0.78 - 50 ng/mL,種交差性:Human,測定試料:Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL),検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC182,測定時間:4.5 hours,測定因子:VAP-1/AOC3
別名:amine oxidase
Genbank No: 8639
法規制等 カルタヘナ該当品
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Quantikine Human VAP-1 ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity
0.077 ng/mL
Assay Range
0.781 - 50 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant human VAP-1
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Human VAP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VAP-1 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VAP-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VAP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VAP-1.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 3.41 10.6 22.7 3.96 11.8 24.6
Standard Deviation 0.071 0.163 0.535 0.18 0.567 1.16
CV% 2.1 1.5 2.4 4.5 4.8 4.7

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Recovery

The recovery of VAP-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 95-114

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Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of VAP-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Additional Infomation

Molecule Information
VAP-1/AOC3
Aliases
AOC3; HPAO; SSAO
Entrez Gene IDs
8639 (Human); 11754 (Mouse); 29473 (Rat)
Background
VAP-1
VAP-1 is a type II transmembrane glycoprotein. It is expressed on endothelium, vascular smooth muscle, and adipocytes. The extracellular domain mediates lymphocyte rolling, adhesion, possibly extravasation during inflammation, and also possesses monoamine oxidase activity. Adipocytes can release a soluble form via metalloproteinase cleavage.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

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