Human Renin Quantikine ELISA Kit | h Renin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111182
R&D Systems製のHuman Reninを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月25日 00時00分現在]
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Human Renin Quantikine ELISA Kit |
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[在庫・価格 ：2024年04月25日 00時00分現在]
Human Renin Quantikine ELISA Kit
文献数： 1
- 商品コード：DREN00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：14.8 pg/mL，測定範囲：31.30 - 2000 pg/mL，種交差性：Human，測定試料：Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (50 uL)，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC154，測定時間：4.5 hours，測定因子：Renin 別名：angiotensin-forming enzyme Genbank No： 5972 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Human Renin Quantikine(R) ELISA Kit Quantikine Human Renin ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (50 µL)
- Sensitivity
- 14.8 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Renin. This assay recognizes both the Pro- and mature versions of recombinant human Renin.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Renin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human mature Renin and Prorenin in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human Renin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Renin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Renin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 201 | 599 | 1,193 | 191 | 566 | 1,153 |
| Standard Deviation | 10.6 | 14.2 | 19.9 | 10.5 | 27.1 | 46.4 |
| CV% | 5.3 | 2.4 | 1.7 | 5.5 | 4.8 | 4 |
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Recovery
The recovery of Renin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=8) | 100 | 93-106 |
| Serum (n=4) | 101 | 90-109 |
| EDTA Plasma (n=4) | 101 | 95-107 |
| Heparin Plasma (n=4) | 105 | 99-114 |
| Urine (n=4) | 93 | 80-106 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Renin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Renin
- Aliases
- Angiotensinogenase; REN
- Entrez Gene IDs
- 5972 (Human); 19701 (Mouse)
- Background
- Renin and Receptor
- Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. The 406 aa precursor includes a signal peptide (aa 1 - 23), a propeptide (aa 24 - 66), and a mature chain (aa 67 - 406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat.Mouse Renin is a secreted, 42 - 47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 - 402). In the kidney, prorenin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-renin shares 70% and 85% aa sequence identity with human and rat pro-renin, respectively.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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