Human IL-1ra/IL-1F3 Quantikine ELISA Kit | hIL-1ra QKit
掲載日情報：2016/08/18 現在Webページ番号：111180
R&D Systems製のHuman IL-1ra/IL-1F3を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月09日 14時55分現在]
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IL-1ra/IL-1F3, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年05月09日 14時55分現在]
IL-1ra/IL-1F3, Human, ELISA Kit, Quantikine (96well)
文献数： 86
- 商品コード：DRA00B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：18.3 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，別売品コントロール：#QC25，測定時間：4.5 hours，測定因子：IL-1ra, IL-1F3 別名：DIRA Genbank No： 3557 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 18.3 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-1ra
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1ra showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 66.9 | 607 | 1,627 | 82.5 | 646 | 1,663 |
| Standard Deviation | 4.88 | 30.2 | 60 | 9.04 | 51.4 | 111 |
| CV% | 7.3 | 5 | 3.7 | 11 | 8 | 6.7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 64.9 | 538 | 1,343 | 56.2 | 469 | 1,243 |
| Standard Deviation | 3.67 | 22.1 | 55.7 | 5.79 | 31.9 | 70.4 |
| CV% | 5.6 | 4.1 | 4.1 | 10.3 | 6.8 | 5.7 |
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Recovery
The recovery of IL-1ra spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 106 | 96-115 |
| Serum (n=4) | 90 | 81-99 |
| EDTA Plasma (n=4) | 86 | 66-102 |
| Heparin Plasma (n=4) | 93 | 82-106 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of IL-1ra were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-1ra/IL-1F3
- Aliases
- IL-1F3; IL1RN
- Entrez Gene IDs
- 3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
- Background
- IL-1ra/IL-1F3
- IL-1 receptor antagonist (IL-1ra) was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1RII does not bind IL-1ra.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Altered monocyte activation markers in Tourette's syndrome: a case-control study.
Dehning S, Gruber R, Krause DL, Matz J, Muller N, Riedel M, Schwarz MJ
BMC Psychiatry 2012 12:29
Species: Human
Sample Type: Serum
Opium addiction increases interleukin 1 receptor antagonist (IL-1Ra) in the coronary artery disease patients.
Dabbagh , Ali, Ghanemnia , Maryam, Gholami , Omid, Namazi , Mohammad, Saadat , Habiboll, Safi , Morteza, Vakili , Hosein, Ziai , Seyed Al
PLoS ONE 2012 7:e44939
Species: Human
Sample Type: Plasma
Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
Barbiche E, Bieuzen F, Fillard JR, Hausswirth C, Louis J, Pournot H
PLoS ONE 2011 6:e22748
Species: Human
Sample Type: Plasma
SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
Auwerx J, Elliott P, Itkonen P, Kainulainen S, Laakso M, Lagouge M, Modi S, Pihlajamaki J, Rutanen J, Sinclair DA, Vanttinen M, Westphal C, Yaluri N, Yamamoto H
Diabetes 2010 59:829-35
Species: Human
Sample Type: Plasma
Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
Laakso M, Pihlajamaki J, Punnonen K, Ruotsalainen E, Salmenniemi U, Stancakova A, Vauhkonen I
Atherosclerosis 2010 210:536-41
Species: Human
Sample Type: Plasma
Accelerated increase in serum interleukin-1 receptor antagonist starts 6 years before diagnosis of type 2 diabetes: Whitehall II prospective cohort study.
Brunner EJ, Carstensen M, Herder C, Jokela M, Kivimaki M, Roden M, Shipley MJ, Tabak AG, Witte DR
Diabetes 2010 59:1222-7
Species: Human
Sample Type: Serum
The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
Berthier CC, Kaplan MJ, Kretzler M, Mattinzoli D, Rastaldi MP, Thacker SG
J. Immunol. 2010 185:4457-69
Species: Human
Sample Type: Serum
Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.
Garcia CA, Kinane DF, Martin M, Rehani K, Wang H
J. Immunol. 2009 182:547-53
Species: Human
Sample Type: Cell Culture Supernates
IL-1 mediates pulmonary and systemic inflammatory responses to chorioamnionitis induced by lipopolysaccharide.
Cheah FC, Ikegami M, Jobe AH, Kallapur SG, Kramer BW, Moss TJ, Newnham JP, Nitsos I, Pillow JJ, Polglase GR
Am. J. Respir. Crit. Care Med. 2009 179:955-61
Species: Ovine
Sample Type: Amniotic Fluid
In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
Bek S, Bosset S, Felix SD, Hartmann N, Hawkins PN, Jung T, Lachmann HJ, Leslie K, Lowe P, Madhoo S, Rordorf C, Wittkowski H
J. Exp. Med. 2009 206:1029-36
Species: Human
Sample Type: Plasma
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