Human IL-6 R alpha Quantikine ELISA Kit | hIL-6 Ra QKit
掲載日情報：2016/08/18 現在Webページ番号：111179
R&D Systems製のHuman IL-6 R alphaを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月05日 00時00分現在]
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IL-6 Receptor α, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月05日 00時00分現在]
IL-6 Receptor α, Human, ELISA Kit, Quantikine (96well)
文献数： 68
- 商品コード：DR600
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：15.1 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC05，測定時間：4.5 hours，測定因子：IL-6R alpha 別名：CD126，IL-6Ra，IL6R，IL-6R alpha，IL-6R α Genbank No： 3570 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 |
Quantikine IL-6 ELISA Kit Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL), Urine (50 µL)
- Sensitivity
- 15.1 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human IL-6 sR
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IL-6 sR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-6 sR in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 sR produced in Sf 21 cells and antibodies raised against this protein. This kit has been shown to accurately quantitate the recombinant soluble IL-6 R. Results obtained using natural IL-6 sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-6 sR. Since the measurement of human IL-6 sR by this immunoassay is insensitive to added recombinant human IL-6, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples ( i.e. the total amount of free receptor plus the total amount of receptor bound to IL-6).
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 134 | 644 | 1,669 | 130 | 645 | 1,593 |
| Standard Deviation | 11.5 | 16.8 | 38.3 | 8.3 | 26.9 | 75.4 |
| CV% | 8.6 | 2.6 | 2.3 | 6.4 | 4.2 | 4.7 |
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Recovery
The recovery of IL-6 sR spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 93 | 86-110 |
| Heparin Plasma (n=4) | 101 | 87-118 |
| Citrate Plasma (n=4) | 93 | 86-102 |
| EDTA Plasma (n=4) | 93 | 76-107 |
| Urine (n=4) | 86 | 64-98 |
| Cell Culture Media (n=4) | 93 | 85-97 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of natural or recombinant human IL-6 sR were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the as
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Additional Infomation
- Molecule Information
- IL-6 R alpha
- Aliases
- CD126; IL6R
- Entrez Gene IDs
- 3570 (Human); 16194 (Mouse)
- Background
- IL-6 Receptor alpha
- The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R) and a signal-transducing component of 130 kDa (gp 130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans. This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alphas have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Inflammatory markers and the risk of hip fracture: the Women's Health Initiative.
Allison MA, Barbour KE, Bauer DC, Boudreau R, Cauley JA, Danielson ME, Greep NC, Jackson RD, LaCroix AZ, Wactawski-Wende J, Wallace RB, Youk AO
J. Bone Miner. Res. 2012 27:1167-76
Species: Human
Sample Type: Serum
Bruton tyrosine kinase inhibition is a novel therapeutic strategy targeting tumor in the bone marrow microenvironment in multiple myeloma.
Acharya C, Anderson K, Buggy J, Cagnetta A, Calle Y, Carrasco D, Cea M, Chang B, Elias L, Fulciniti M, Hu Y, Kong S, Lin J, Matsui W, Munshi N, Richardson P, Sellitto M, Tai Y, Treon S, Wang Q, Yang G, Zhao J, Zhong M
Blood 2012 120:1877-87
Species: Human
Sample Type: recombinant IL-6 R in mouse serum
Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
Amoudruz P, Doumbo O, Doumbo S, Haileselassie Y, Holmlund U, Johansson MA, Kayentao K, Montgomery SM, Ongoiba A, Schollin J, Sverremark-Ekstrom E, Traore B
Clin. Exp. Immunol. 2010 162:500-9
Species: Human
Sample Type: Milk
Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
Ara T, DeClerck YA, Groshen SG, Keshelava N, Metelitsa LS, Russell HV, Seeger RC, Shimada H, Song L
Cancer Res. 2009 69:329-37
Species: Human
Sample Type: Serum
Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease.
Abuin I, Almeida J, Cuello R, De Las Heras N, Dominguez M, Galende J, Hernandez J, Martin-Ayuso M, Martin-Nunez G, Mateo G, Moreno I, Moro MJ, Orfao A, Perez-Andres M, San Miguel JF
Int. J. Cancer 2009 124:367-75
Species: Human
Sample Type: Plasma
Daily family assistance and inflammation among adolescents from Latin American and European backgrounds.
Bower J, Cole SW, Fuligni AJ, Irwin MR, Kiang L, Telzer EH
Brain Behav. Immun. 2009 23:803-9
Species: Human
Sample Type: Plasma
Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
Anderson KC, Bandi M, Bianchi G, Brahmandam M, Carrasco R, Chauhan D, Hideshima T, Jaye DL, Kumar SK, Mitsiades C, Munshi N, Podar K, Raje N, Richardson P, Singh AV, Tai YT
Cancer Cell 2009 16:309-23
Species: Human
Sample Type: Serum
Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
Daniel V, Lahdou I, Naujokat C, Opelz G, Sadeghi M, Schnitzler P, Zeier M
Transplantation 2009 88:1109-16
Species: Human
Sample Type: Urine
Improved prediction of disease relapse after radical prostatectomy through a panel of preoperative blood-based biomarkers.
Karakiewicz PI, Karam JA, Margulis V, Montorsi F, Roehrborn CG, Saad F, Shariat SF, Slawin KM, Walz J
Clin. Cancer Res. 2008 14:3785-91
Species: Human
Sample Type: Plasma
Interleukin-6 and soluble interleukin-6 receptor levels as markers of disease extent and prognosis in neuroblastoma.
Burlingame SM, Egler RA, Nuchtern JG, Russell HV
Clin. Cancer Res. 2008 14:7028-34
Species: Human
Sample Type: Plasma
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