Human Nectin-4 Quantikine ELISA Kit | hNectin-4 QKit
掲載日情報：2016/08/18 現在Webページ番号：111159
R&D Systems製のHuman Nectin-4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月04日 00時00分現在]
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Human Nectin-4 Quantikine ELISA Kit |
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[在庫・価格 ：2025年05月04日 00時00分現在]
Human Nectin-4 Quantikine ELISA Kit
文献数： 1
- 商品コード：DNEC40
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別売品コントロール：QC133，感度：16.6 pg/mL，測定範囲：39.1-2,500 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Tissue Lysate，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，測定時間：4.5 hours，測定因子：Nectin-4 別名：EDSS1 Genbank No： 81607 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Human Nectin-4 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (25 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (13 µL)
- Sensitivity
- 16.6 pg/mL
- Assay Range
- 39.1 - 2,500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Nectin-4.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Nectin-4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Nectin-4 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human Nectin-4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Nectin-4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Nectin-4.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 163 | 425 | 1,365 | 194 | 474 | 1,440 |
| Standard Deviation | 5.56 | 13.9 | 32.7 | 15.8 | 27.9 | 65.8 |
| CV% | 3.4 | 3.3 | 2.4 | 8.1 | 5.9 | 4.6 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 232 | 603 | 1,867 | 245 | 595 | 1,801 |
| Standard Deviation | 7.19 | 37.7 | 40.1 | 18.9 | 31.9 | 82.3 |
| CV% | 3.1 | 6.3 | 2.1 | 7.7 | 5.4 | 4.6 |
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Recovery
The recovery of human Nectin-4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 100 | 94-110 |
| Serum (n=4) | 90 | 80-101 |
| EDTA Plasma (n=4) | 89 | 80-109 |
| Heparin Plasma (n=4) | 91 | 82-99 |
| Urine (n=4) | 100 | 93-108 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Nectin-4 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Nectin-4
- Aliases
- LNIR; PRR4; PVRL4
- Entrez Gene IDs
- 81607 (Human); 71740 (Mouse)
- Background
- Nectin-4
- Nectins are type I transmembrane glycoproteins that are calcium-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs). Homology has led to their designation as poliovirus receptor-related (PRR) proteins, but nectins do not bind poliovirus. The extracellular domains of the nectin family form cis-homodimers (same cell), followed by homophilic or heterophilic trans-dimers (across cells) that organize intercellular junctions. Nectin-1, also known as CD111, PRR1 and herpes virus entry mediator C (HVEC) has a secreted splice variant. Nectin-1, Nectin-2 (CD112, PRR2, HVEB) and Nectin-3 (PRR3) are concentrated in adherens junctions and exist on neurons, endothelial cells, epithelial cells and fibroblasts.Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the placenta, the embryo, and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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