Human LIGHT/TNFSF14 Quantikine ELISA Kit | hLIGHT/TNFSF14 QKit

掲載日情報:2016/08/18 現在Webページ番号:111137

R&D Systems製のHuman LIGHT/TNFSF14を測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2020年07月13日 11時51分現在]

※ 納期として表示している期間は弊社発注日からの「目安」となり、納期を保証する物ではございません。実際には長くかかる場合もございます。ご注意ください。
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納期 文献数
LIGHT/TNFSF14, Human, ELISA Kit, Quantikine (96 well)
1週間程度  11
説明文
測定範囲:31.20 - 2000 pg/mL,感度:16.5 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL),種交差性:Human,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC114,測定時間:4.5 hours,測定因子:LIGHT/TNFSF14
別名:CD258
Genbank No: 8740
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2017年3月1日号 p.6

製品記事
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[在庫・価格 :2020年07月13日 11時51分現在]

※ 納期として表示している期間は弊社発注日からの「目安」となり、納期を保証する物ではございません。実際には長くかかる場合もございます。ご注意ください。

LIGHT/TNFSF14, Human, ELISA Kit, Quantikine (96 well)

文献数:11

  • 商品コード:DLIT00
  • メーカー:RSD
  • 包装:1kit
  • 価格:¥96,000
  • 在庫:無(未発注)
  • 納期:1週間程度 
  • 法規制等:

説明文 測定範囲:31.20 - 2000 pg/mL,感度:16.5 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL),種交差性:Human,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC114,測定時間:4.5 hours,測定因子:LIGHT/TNFSF14
別名:CD258
Genbank No: 8740
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2017年3月1日号 p.6

製品記事
関連記事

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (25 µL)

Sensitivity
16.5 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant human LIGHT
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Human LIGHT Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LIGHT in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human LIGHT and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LIGHT showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LIGHT.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 308 626 1,181 318 616 1,177
Standard Deviation 11.7 25.3 34.6 27 44.2 81.8
CV% 3.8 4 2.9 8.5 7.2 6.9
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 350 712 1,388 387 754 1,469
Standard Deviation 12 31.7 63.3 19.6 31.8 63.4
CV% 3.4 4.5 4.6 5.1 4.2 4.3

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Recovery

The recovery of LIGHT spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=5) 102 85-114
Heparin Plasma (n=5) 101 89-115
EDTA Plasma (n=5) 106 91-114
Cell Culture Media (n=4) 105 100-113

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Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of LIGHT were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Additional Infomation

Molecule Information
LIGHT/TNFSF14
Aliases
CD258; TNFSF14
Entrez Gene IDs
8740 (Human); 50930 (Mouse)
Background
LIGHT/TNFSF14
Human LIGHT is a type II membrane protein that is a member of the TNF superfamily (TNFSF14). LIGHT is an acronym that stands for is homologous to Lymphotoxins, exhibits Inducible expression, and competes with HSV Glycoprotein D for HVEM, a receptor expressed by Tlymphocytes. LIGHT has also been called HVEM-L and LT-gamma.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
Aukrust P, Froland SS, Landro L, Otterdal K, Ueland T
J. Thromb. Haemost. 2011 9:1075-7
Species: Human
Sample Type: Platelet-Poor Plasma


Adenovirus-mediated intratumoral expression of immunostimulatory proteins in combination with systemic Treg inactivation induces tumor-destructive immune responses in mouse models.
Beyer I, Hellstrom KE, Li ZY, Lieber A, Liu Y, Persson J, Roffler S, Tragoolpua K, Tuve S, Yumul R
Cancer Gene Ther. 2011 18:407-18
Species: Human
Sample Type: Cell Culture Supernates


LIGHT is associated with hypertriglyceridemia in obese subjects and increased cytokine secretion from cultured human adipocytes.
Bassols J, Moreno-Navarrete JM, Ortega F
Int J Obes (Lond) 2010 34:146-56
Species: Human
Sample Type: Serum


Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
Aukrust P, Brosstad FR, Damas JK, Froland SS, Gullestad L, Halvorsen B, Hansson GK, Oie E, Olofsson PS, Otterdal K, Sandberg WJ, Smith C, Yndestad A
Circ. Res. 2009 104:60-8
Species: Human
Sample Type: Cell Culture Supernates


Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
Mabilleau G, Sabokbar A
PLoS ONE 2009 4:e4173
Species: Human
Sample Type: Cell Culture Supernates


The lymphotoxin-beta receptor is an upstream activator of NF-kappaB-mediated transcription in melanoma cells.
Baugher P, Cheung TC, Dhawan P, Ellis DL, Kelley M, Richmond A, Sobolik-Delmaire T, Su Y, Thu YM, Ware CF, Yu Y
J. Biol. Chem. 2008 283:15399-408
Species: Human
Sample Type: Cell Culture Supernates


Enhanced plasma levels of LIGHT in patients with acute atherothrombotic stroke.
Fang LB, Gao XG, Hjelmstrom P, Liu GZ
Acta Neurol. Scand. 2008 118:256-9
Species: Human
Sample Type: Plasma


Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium.
Celik S, Dengler TJ, Gawaz MP, Katus HA, Kurz K, Langer H, May AE, Shankar V, Stellos K
Thromb. Haemost. 2007 98:798-805
Species: Human
Sample Type: Cell Culture Supernates


LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
Adamopoulos IE, Athanasou NA, Edwards JR, Locklin R, Sabokbar A, Shipman CM, Sun SG
Arthritis Rheum. 2006 54:1451-62
Species: Human
Sample Type: Serum


Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Aukrust P, Damas JK, Endresen K, Oie E, Otterdal K, Pedersen TM, Smith C, Solum NO, Stang E, Yndestad A
Blood 2006 108:928-35
Species: Human
Sample Type: Plasma


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