Human LIGHT/TNFSF14 Quantikine ELISA Kit | hLIGHT/TNFSF14 QKit
掲載日情報：2016/08/18 現在Webページ番号：111137
R&D Systems製のHuman LIGHT/TNFSF14を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月26日 20時55分現在]
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LIGHT/TNFSF14, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2024年04月26日 20時55分現在]
LIGHT/TNFSF14, Human, ELISA Kit, Quantikine (96 well)
文献数： 12
- 商品コード：DLIT00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：16.5 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC114，測定時間：4.5 hours，測定因子：LIGHT/TNFSF14 別名：CD258 Genbank No： 8740 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2020年10月1日号 p.25
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| 製品記事 |
Immune Checkpoint Protein ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (25 µL)
- Sensitivity
- 16.5 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human LIGHT
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human LIGHT Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LIGHT in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human LIGHT and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LIGHT showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LIGHT.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 308 | 626 | 1,181 | 318 | 616 | 1,177 |
| Standard Deviation | 11.7 | 25.3 | 34.6 | 27 | 44.2 | 81.8 |
| CV% | 3.8 | 4 | 2.9 | 8.5 | 7.2 | 6.9 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 350 | 712 | 1,388 | 387 | 754 | 1,469 |
| Standard Deviation | 12 | 31.7 | 63.3 | 19.6 | 31.8 | 63.4 |
| CV% | 3.4 | 4.5 | 4.6 | 5.1 | 4.2 | 4.3 |
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Recovery
The recovery of LIGHT spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 102 | 85-114 |
| Heparin Plasma (n=5) | 101 | 89-115 |
| EDTA Plasma (n=5) | 106 | 91-114 |
| Cell Culture Media (n=4) | 105 | 100-113 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of LIGHT were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- LIGHT/TNFSF14
- Aliases
- CD258; TNFSF14
- Entrez Gene IDs
- 8740 (Human); 50930 (Mouse)
- Background
- LIGHT/TNFSF14
- Human LIGHT is a type II membrane protein that is a member of the TNF superfamily (TNFSF14). LIGHT is an acronym that stands for is homologous to Lymphotoxins, exhibits Inducible expression, and competes with HSV Glycoprotein D for HVEM, a receptor expressed by Tlymphocytes. LIGHT has also been called HVEM-L and LT-gamma.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
Aukrust P, Froland SS, Landro L, Otterdal K, Ueland T
J. Thromb. Haemost. 2011 9:1075-7
Species: Human
Sample Type: Platelet-Poor Plasma
Adenovirus-mediated intratumoral expression of immunostimulatory proteins in combination with systemic Treg inactivation induces tumor-destructive immune responses in mouse models.
Beyer I, Hellstrom KE, Li ZY, Lieber A, Liu Y, Persson J, Roffler S, Tragoolpua K, Tuve S, Yumul R
Cancer Gene Ther. 2011 18:407-18
Species: Human
Sample Type: Cell Culture Supernates
LIGHT is associated with hypertriglyceridemia in obese subjects and increased cytokine secretion from cultured human adipocytes.
Bassols J, Moreno-Navarrete JM, Ortega F
Int J Obes (Lond) 2010 34:146-56
Species: Human
Sample Type: Serum
Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
Aukrust P, Brosstad FR, Damas JK, Froland SS, Gullestad L, Halvorsen B, Hansson GK, Oie E, Olofsson PS, Otterdal K, Sandberg WJ, Smith C, Yndestad A
Circ. Res. 2009 104:60-8
Species: Human
Sample Type: Cell Culture Supernates
Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
Mabilleau G, Sabokbar A
PLoS ONE 2009 4:e4173
Species: Human
Sample Type: Cell Culture Supernates
The lymphotoxin-beta receptor is an upstream activator of NF-kappaB-mediated transcription in melanoma cells.
Baugher P, Cheung TC, Dhawan P, Ellis DL, Kelley M, Richmond A, Sobolik-Delmaire T, Su Y, Thu YM, Ware CF, Yu Y
J. Biol. Chem. 2008 283:15399-408
Species: Human
Sample Type: Cell Culture Supernates
Enhanced plasma levels of LIGHT in patients with acute atherothrombotic stroke.
Fang LB, Gao XG, Hjelmstrom P, Liu GZ
Acta Neurol. Scand. 2008 118:256-9
Species: Human
Sample Type: Plasma
Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium.
Celik S, Dengler TJ, Gawaz MP, Katus HA, Kurz K, Langer H, May AE, Shankar V, Stellos K
Thromb. Haemost. 2007 98:798-805
Species: Human
Sample Type: Cell Culture Supernates
LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
Adamopoulos IE, Athanasou NA, Edwards JR, Locklin R, Sabokbar A, Shipman CM, Sun SG
Arthritis Rheum. 2006 54:1451-62
Species: Human
Sample Type: Serum
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Aukrust P, Damas JK, Endresen K, Oie E, Otterdal K, Pedersen TM, Smith C, Solum NO, Stang E, Yndestad A
Blood 2006 108:928-35
Species: Human
Sample Type: Plasma
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