Human LAP (TGF-beta 1) Quantikine ELISA Kit | hLAP (TGF-b1) QKit
掲載日情報：2016/08/18 現在Webページ番号：111131
R&D Systems製のHuman LAP (TGF-beta 1)を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月30日 00時00分現在]
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[在庫・価格 ：2024年04月30日 00時00分現在]
Human LAP (TGF-beta 1) Quantikine ELISA Kit
文献数： 0
- 商品コード：DLAP00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：3.4 pg/mL，測定範囲：23.40 - 1500 pg/mL，測定試料：Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL)，種交差性：Human，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Sandwich，アッセイ数：96，別売品コントロール：QC110，測定時間：4.5 hours，測定因子：LAP (TGF-beta 1) 別名：LAP (TGFbeta 1) Genbank No： 7040 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human LAP(TGF-β1) ELISA Kit Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL), Urine (50 µL)
- Sensitivity
- 3.4 pg/mL
- Assay Range
- 23.4 - 1,500 pg/mL (Serum, Cell Culture Supernates, Cell Lysates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
- Specificity
- Natural and recombinant human LAP (TGF-beta 1) and Latent TGF-beta 1).
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human LAP (TGF-beta 1) Immunoassay is a 4.5 hour solid phase ELISA designed to measure LAP (TGF-beta 1) in acid activated cell culture supernates, serum, plasma, and urine. It contains recombinant human LAP (TGF-beta 1) expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural LAP (TGF-beta 1) showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural human LAP (TGF-beta 1).
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 218 | 427 | 992 | 216 | 460 | 1,126 |
| Standard Deviation | 6.92 | 17.7 | 42.1 | 7.68 | 21.7 | 86.9 |
| CV% | 3.2 | 4.1 | 4.2 | 3.6 | 4.7 | 7.7 |
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Recovery
The recovery of LAP (TGF-beta 1) spiked to levels throughout the range of the assay followed by activation was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 98 | 90-105 |
| Cell Lysates (n=4) | 97 | 90-103 |
| Urine (n=4) | 95 | 87-101 |
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Linearity
To assess the linearity of the assay, activated samples containing and/or spiked with high concentrations of LAP (TGF-beta 1) were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- LAP (TGF-beta 1)
- Aliases
- undefined
- Entrez Gene IDs
- 7040 (Human)
- Background
- TGF-beta Family Ligands
- Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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