Human Galectin-9 Quantikine ELISA Kit | hGalectin-9 Qkit

掲載日情報:2016/08/18 現在Webページ番号:111100

R&D Systems製のHuman Galectin-9を測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2020年06月01日 19時51分現在]

※ 納期として表示している期間は弊社発注日からの「目安」となり、納期を保証する物ではございません。実際には長くかかる場合もございます。ご注意ください。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Galectin-9, Human, ELISA Kit, Quantikine
- 5
説明文
測定範囲:0.16 - 10 ng/mL,感度:0.028 ng/mL,測定試料:Cell Culture Supernates (100 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (100 uL), Urine (10 uL), Human Milk (10 uL),種交差性:Human,測定波長:450 nm,検出方法:colorimetric,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96,別売品コントロール:QC96,測定時間:4.5 hours,測定因子:Galectin-9
別名:ecalectin
Genbank No: 3965
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2019年8月15日号 p.6
ニュース2018年7月1日号 p.6

製品記事 Quantikine Human Galectin-9 ELISA Kit
関連記事

[在庫・価格 :2020年06月01日 19時51分現在]

※ 納期として表示している期間は弊社発注日からの「目安」となり、納期を保証する物ではございません。実際には長くかかる場合もございます。ご注意ください。

Galectin-9, Human, ELISA Kit, Quantikine

文献数:5

  • 商品コード:DGAL90
  • メーカー:RSD
  • 包装:1kit
  • 価格:¥98,000
  • 在庫:1個
  • 納期:-
  • 法規制等:

説明文 測定範囲:0.16 - 10 ng/mL,感度:0.028 ng/mL,測定試料:Cell Culture Supernates (100 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (100 uL), Urine (10 uL), Human Milk (10 uL),種交差性:Human,測定波長:450 nm,検出方法:colorimetric,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96,別売品コントロール:QC96,測定時間:4.5 hours,測定因子:Galectin-9
別名:ecalectin
Genbank No: 3965
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2019年8月15日号 p.6
ニュース2018年7月1日号 p.6

製品記事 Quantikine Human Galectin-9 ELISA Kit
関連記事

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (100 µL), Urine (10 µL), Human Milk (10 µL)

Sensitivity
0.028 ng/mL
Assay Range
0.156 - 10 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
Specificity
Natural and recombinant human Galectin-9.
Interference
No significant interference observed with available related molecules.

目次に戻る

Product Summary

The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.

目次に戻る

Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Saliva, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.34 3.58 6.13 1.44 3.43 6.05
Standard Deviation 0.05 0.12 0.22 0.08 0.18 0.32
CV% 3.7 3.4 3.6 5.6 5.2 5.3
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.44 3.77 6.29 1.4 3.28 5.84
Standard Deviation 0.06 0.07 0.08 0.11 0.2 0.3
CV% 4.2 1.9 1.3 7.9 6.1 5.1

目次に戻る

Recovery

The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 84-104
Serum (n=4) 106 86-117
EDTA Plasma (n=4) 103 85-117
Heparin Plasma (n=4) 104 89-117

目次に戻る

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

目次に戻る

Additional Infomation

Molecule Information
Galectin-9
Aliases
Ecalectin; GAL9; LGALS9
Entrez Gene IDs
3965 (Human); 16859 (Mouse)
Background
Galectins
The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

目次に戻る

Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。