Human Galectin-9 Quantikine ELISA Kit | hGalectin-9 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111100
R&D Systems製のHuman Galectin-9を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月27日 00時00分現在]
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Galectin-9, Human, ELISA Kit, Quantikine |
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[在庫・価格 ：2024年04月27日 00時00分現在]
Galectin-9, Human, ELISA Kit, Quantikine
文献数： 9
- 商品コード：DGAL90
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.16 - 10 ng/mL，感度：0.028 ng/mL，測定試料：Cell Culture Supernates (100 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (100 uL), Urine (10 uL), Human Milk (10 uL)，種交差性：Human，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC96，測定時間：4.5 hours，測定因子：Galectin-9 別名：ecalectin Genbank No： 3965 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human Galectin-9 ELISA Kit R＆D Systems社のGalectin-9関連製品 Immune Checkpoint Protein ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (100 µL), Urine (10 µL), Human Milk (10 µL)
- Sensitivity
- 0.028 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Galectin-9.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Saliva, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.34 | 3.58 | 6.13 | 1.44 | 3.43 | 6.05 |
| Standard Deviation | 0.05 | 0.12 | 0.22 | 0.08 | 0.18 | 0.32 |
| CV% | 3.7 | 3.4 | 3.6 | 5.6 | 5.2 | 5.3 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.44 | 3.77 | 6.29 | 1.4 | 3.28 | 5.84 |
| Standard Deviation | 0.06 | 0.07 | 0.08 | 0.11 | 0.2 | 0.3 |
| CV% | 4.2 | 1.9 | 1.3 | 7.9 | 6.1 | 5.1 |
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Recovery
The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 96 | 84-104 |
| Serum (n=4) | 106 | 86-117 |
| EDTA Plasma (n=4) | 103 | 85-117 |
| Heparin Plasma (n=4) | 104 | 89-117 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Galectin-9
- Aliases
- Ecalectin; GAL9; LGALS9
- Entrez Gene IDs
- 3965 (Human); 16859 (Mouse)
- Background
- Galectins
- The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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