Human Galectin-3 Quantikine ELISA Kit | hGalectin-3 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111099
R&D Systems製のHuman Galectin-3を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年07月17日 09時55分現在]
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Galectin-3, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年07月17日 09時55分現在]
Galectin-3, Human, ELISA Kit, Quantikine (96well)
文献数： 18
- 商品コード：DGAL30
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.313-10 ng/mL，感度：0.085 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC24，測定時間：4.5 hours，測定因子：Galectin-3 別名：AGE-R3 Genbank No： 3958 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Galectin-3 Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 0.085 ng/mL
- Assay Range
- 0.313 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human Galectin-3
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Galectin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Galectin-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Galectin-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.78 | 2.27 | 5.06 | 0.81 | 2.51 | 5.29 |
| Standard Deviation | 0.03 | 0.1 | 0.15 | 0.07 | 0.2 | 0.36 |
| CV% | 3.8 | 4.4 | 3 | 8.6 | 8 | 6.8 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.79 | 2.46 | 5.11 | 0.8 | 2.59 | 5.5 |
| Standard Deviation | 0.03 | 0.09 | 0.18 | 0.05 | 0.16 | 0.32 |
| CV% | 3.8 | 3.7 | 3.5 | 6.3 | 6.2 | 5.8 |
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Recovery
The recovery of Galectin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 104 | 94-115 |
| Heparin Plasma (n=4) | 103 | 95-110 |
| EDTA Plasma (n=4) | 105 | 93-113 |
| Cell Culture Media (n=4) | 102 | 96-106 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of human Galectin-3 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Galectin-3
- Aliases
- AGE-R3; CBP35; GAL3; L29; LGALS3; Mac-2
- Entrez Gene IDs
- 3958 (Human); 16854 (Mouse)
- Background
- Galectins
- The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Hypoxia potentiates glioma-mediated immunosuppression.
Fokt I, Fuller G, Heimberger AB, Kong LY, Melillo G, Priebe W, Wang Y, Wei J, Wu A
PLoS ONE 2012 6:e16195
Species: Human
Sample Type: Cell Culture Supernates
Galectin-3 in cord blood of term and preterm infants.
Bohlmann MK, Demmert M, Faust K, Gopel W, Hartel C, Herting E, Troger B
Clin. Exp. Immunol. 2012 167:246-51
Species: Human
Sample Type: Cell Culture Supernates
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